View : 225 Download: 0

Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli

Title
Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli
Authors
Yang, Jung EunKim, Je WoongOh, Young HoonChoi, So YoungLee, HyukPark, A-ReumShin, JihoonPark, Si JaeLee, Sang Yup
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
BIOTECHNOLOGY JOURNAL
ISSN
1860-6768JCR Link

1860-7314JCR Link
Citation
BIOTECHNOLOGY JOURNAL vol. 11, no. 12, pp. 1572 - 1585
Keywords
Escherichia coli2-HydroxyacidsMetabolic engineeringPolyhydroxyalkanoatesPoly(2-hydroxyisovalerate-co-lactate)
Publisher
WILEY-V C H VERLAG GMBH
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Polyhydroxyalkanoates (PHAs) containing 2-hydroxyacids such as lactate (LA) and 2-hydroxybutyrate (2HB) have recently been produced by metabolically engineered microorganisms. Here, we further expanded 2-hydroxyacid monomer spectrum of PHAs by engineering Escherichia coli to produce PHAs containing 2-hydroxyisovalerate (2HIV). To generate 2HIV in vivo, feedback resistant ilvBN(mut) genes encoding acetohydroxyacid synthase and ilvCD genes encoding ketol-acid reductoisomerase and dihydroxyacid dehydratase, respectively, and panE gene encoding D-2-hydroxyacid dehydrogenase are overexpressed. Also, pct540 gene encoding evolved propionyl-CoA transferase and phaC1437 gene encoding evolved PHA synthase are overexpressed along with ilvBN(mut), ilvCD, and panE genes in E. coli strain for in vivo synthesis of 2HIV containing PHAs. E. coli strain expressing all of these genes can produce poly(13.2 mol% 2HIV-co-7.5 mol% -2HB-co-42.5 mol% 3HB-co-36.8 mol% LA) when it is cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 3-hydroxybutyrate (3HB). To produce PHA containing only 2HIV and LA monomers, poxB, pflB, adhE and frdB genes encoding enzymes involved in competing pathways for pyruvate are deleted so that cells can generate more 2HIV and LA. When this engineered E. coli strain expressing ilvBN(mut), ilvCD, panE, pct540 and phaC1437 genes is cultured in the medium containing 20 g/L of glucose and 2 mM L-isoleucine, which can inhibit L-threonine dehydratase responsible for in vivo 2HB generation, poly(20 mol% 2HIV-co-80 mol% LA) can be produced to the polymer content of 9.6% w/w. These results suggest that novel PHAs containing 2HIV can be produced by engineering branched-chain amino acid metabolism.
Show the fulltext
DOI
10.1002/biot.201600420
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE