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Control strategy of displacement processes to sense biothiols via fluorescent changes
- Title
- Control strategy of displacement processes to sense biothiols via fluorescent changes
- Authors
- Kwon N.; Hong J.; Kim G.; Jun E.J.; Yoon J.
- Ewha Authors
- 윤주영
- SCOPUS Author ID
- 윤주영
- Issue Date
- 2020
- Journal Title
- Dyes and Pigments
- ISSN
- 0143-7208
- Citation
- Dyes and Pigments vol. 173
- Keywords
- Biothiol selective probes; Cu2+ selective fluorescent probes; Displacement approach; Fluorescent imaging probes; Fluorescent probe for GSH
- Publisher
- Elsevier Ltd
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Two different bis-pyrene derivatives BP-1 and BP-2 were synthesized, which showed efficient fluorescent quenching by Cu2+. The addition of biothiols, such as GSH, Cys, and Hcy, restored their fluorescent emissions. Differences in the affinities of BP-1 and BP-2 for Cu2+ were successfully applied to the different detection limits and the linear detection range of biothiols. An NH moiety and a sulfur atom in the linker were the only differences between BP-1 and BP-2. Since BP-1 bound Cu2+ more tightly than BP-2, BP-1-Cu2+ required more GSH, Cys, and Hcy equivalents to restore the fluorescent emission. In contrast, only two equivalents of biothiols restored the original emission of BP-2-Cu2+. As a proof-of-concept, we demonstrated that molecular recognition chemistry and host-gest chemistry, more specifically the modulation of metal ion recognition, can be successfully applied to biothiol detection, especially over different concentration ranges. BP-1 showed a fluorescent quenching effect in living cells upon the addition of Cu2+ and BP-1-Cu2+ was also successfully applied to imaging biothiols in living cells. © 2019
- DOI
- 10.1016/j.dyepig.2019.107871
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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