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Therapeutic effects of three human-derived materials in a mouse corneal alkali burn model*
- Title
- Therapeutic effects of three human-derived materials in a mouse corneal alkali burn model*
- Authors
- Han, Kyung Eun; Park, Mi Hye; Kong, Kyoung Hye; Choi, Euno; Choi, Kyu-Ryong; Jun, Roo Min
- Ewha Authors
- 최규룡; 박미혜; 전루민; 공경혜; 한경은; 최은오
- SCOPUS Author ID
- 최규룡; 박미혜; 전루민; 공경혜; 한경은; 최은오
- Issue Date
- 2019
- Journal Title
- CUTANEOUS AND OCULAR TOXICOLOGY
- ISSN
- 1556-9527
1556-9535
- Citation
- CUTANEOUS AND OCULAR TOXICOLOGY vol. 38, no. 4, pp. 315 - 321
- Keywords
- Alkali injury; peripheral blood serum; umbilical cord serum; amniotic membrane suspension
- Publisher
- TAYLOR &
FRANCIS LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Purpose: To compare the therapeutic effects of human derivatives in a mouse alkali burn model. Methods: The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 d: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3 degrees of opacity on days 2, 3, and 7, and corneal neovascularization (NV) areas on day 7 were evaluated. Histologic examination and mRNA expression levels of tumour necrosis factor (TNF)-alpha, interleukin (IL)-6, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 were also evaluated on day 7. Results: The epithelial defect areas in the hUCS group were smaller than those in the control and hPBS groups on day 3 (p < .05, respectively). The epithelial defect areas in the hAM suspension group showed smaller than those in the control and hPBS groups on days 1 and 2 (p < .05, respectively). The degrees of opacity were lower in all treatment groups than that of the saline control group on day 7 (p < .05, respectively). Corneal NV areas were not different among groups on day 7 (p = 0.20). The expression levels of TNF-alpha, IL-6, MMP-8, and MMP-9 mRNA and the infiltration of the inflammatory cells in all treatment groups were lesser than those in the control group on day 7 (p< .05, respectively). Conclusions: All treatments reduced inflammatory reactions and corneal opacity development. Corneal reepithelialization was faster in the hUCS group.
- DOI
- 10.1080/15569527.2019.1573244
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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