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Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model

Title
Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model
Authors
Kim, MinjeongJeong, HaengduengLee, BuhyunCho, YejinYoon, Won KeeCho, AhreumKwon, GuideockNam, Ki TaekHa, HunjooLim, Kyung-Min
Ewha Authors
하헌주임경민김민정
SCOPUS Author ID
하헌주scopus; 임경민scopus; 김민정scopusscopus
Issue Date
2019
Journal Title
BIOMOLECULES & THERAPEUTICS
ISSN
1976-9148JCR Link

2005-4483JCR Link
Citation
BIOMOLECULES & THERAPEUTICS vol. 27, no. 5, pp. 457 - 465
Keywords
LipidsCeramideFree fatty acidDiabetes mellitusSkin barrier function
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Indexed
SCIE; SCOPUS; KCI WOS
Document Type
Article
Abstract
Patients with diabetes mellitus (DM) often suffer from diverse skin disorders, which might be attributable to skin barrier dysfunction. To explore the role of lipid alterations in the epidermis in DM skin disorders, we quantitated 49 lipids (34 ceramides, 14 free fatty acids (FFAs), and cholesterol) in the skin epidermis, liver, and kidneys of db/db mice, a Type 2 DM model, using UPLC-MS/MS. The expression of genes involved in lipid synthesis was also evaluated. With the full establishment of hyperglycemia at the age of 20 weeks, remarkable lipid enrichment was noted in the skin of the db/db mice, especially at the epidermis and subcutaneous fat bed. Prominent increases in the ceramides and FFAs (>3 fold) with short or medium chains (<026) occurred in the skin epidermis (16NS, 18NS, 24NS, 16NDS, 18NDS, 20NDS, 22NDS, 24NDS, C16:1FA, C18:2FA, and C18:1FA) and the liver (16NS, 18NS, 20NS, 24:1NS, 18NDS, 20NDS, 22NDS, C16:1FA, C18:2FA, C18:1FA), whereas those with very long chains were not affected. In the kidney, only slight increases (<3 fold) were observed for 16NS, 18NS, 2ONS, 26NDS, C26FA, and C22:1FA. Consistently, LXR alpha/beta and PPAIR gamma, nuclear receptors promoting lipid synthesis, lipid synthesis enzymes such as elongases 1, 4, and 6, and fatty acid synthase and stearoyl-CoA desaturase were highly expressed in the skin and livers of the db/db mice. Collectively, our study demonstrates an extensive alteration in the skin and systemic lipid profiles of db/db mice, which could contribute to the development of skin disorders in DM.
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DOI
10.4062/biomolther.2018.214
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약학대학 > 약학과 > Journal papers
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