View : 322 Download: 0
Formate-removing inoculum dominated by Methanobacterium congolense supports succinate production from crude glycerol fermentation
- Formate-removing inoculum dominated by Methanobacterium congolense supports succinate production from crude glycerol fermentation
- Kim, Nam Yeun; Lee, Chung Min; Kim, So Young; Kim, Ok Bin
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 46, no. 5, pp. 625 - 634
- Formate-removing inoculum; Methanogen; Methanobacterium congolense; Succinate; Crude glycerol
- SPRINGER HEIDELBERG
- SCIE; SCOPUS
- Document Type
Show the fulltext
- We developed a formate-removing methanogenic inoculum (FRI) to facilitate succinate production from crude glycerol by Escherichia coli. FRI converted formate to methane, thereby enabling glycerol fermentation without additional electron acceptors under neutral pH. FRI was selectively enriched from sludge from the anaerobic digester of the Seonam sewage treatment plant(Seoul); this process was assessed via Illumina sequencing and scanning electron microscopy imaging. Methanobacterium congolense species occupied only 0.3% of the archaea community in the sludge and was enriched to 99.5% in complete FRI, wherein succinate-degrading bacteria were successfully eliminated. Co-culture with FRI improved glycerol fermentation and yielded 7.3mM succinate from 28.7mM crude glycerol, whereby FRI completely converted formate into methane. This study is the first to demonstrate methane production by M. congolense species, using formate. M. congolense-dominated FRI can serve as a renewable facilitator of waste feedstock fermentation and enable the production of commercially important compounds.
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.