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Formate-removing inoculum dominated by Methanobacterium congolense supports succinate production from crude glycerol fermentation

Title
Formate-removing inoculum dominated by Methanobacterium congolense supports succinate production from crude glycerol fermentation
Authors
Kim, Nam YeunLee, Chung MinKim, So YoungKim, Ok Bin
Ewha Authors
김옥빈
SCOPUS Author ID
김옥빈scopus
Issue Date
2019
Journal Title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN
1367-5435JCR Link

1476-5535JCR Link
Citation
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 46, no. 5, pp. 625 - 634
Keywords
Formate-removing inoculumMethanogenMethanobacterium congolenseSuccinateCrude glycerol
Publisher
SPRINGER HEIDELBERG
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
We developed a formate-removing methanogenic inoculum (FRI) to facilitate succinate production from crude glycerol by Escherichia coli. FRI converted formate to methane, thereby enabling glycerol fermentation without additional electron acceptors under neutral pH. FRI was selectively enriched from sludge from the anaerobic digester of the Seonam sewage treatment plant(Seoul); this process was assessed via Illumina sequencing and scanning electron microscopy imaging. Methanobacterium congolense species occupied only 0.3% of the archaea community in the sludge and was enriched to 99.5% in complete FRI, wherein succinate-degrading bacteria were successfully eliminated. Co-culture with FRI improved glycerol fermentation and yielded 7.3mM succinate from 28.7mM crude glycerol, whereby FRI completely converted formate into methane. This study is the first to demonstrate methane production by M. congolense species, using formate. M. congolense-dominated FRI can serve as a renewable facilitator of waste feedstock fermentation and enable the production of commercially important compounds.
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DOI
10.1007/s10295-019-02154-w
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자연과학대학 > 생명과학전공 > Journal papers
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