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Bispecific anti-mPDGFR beta x cotinine scFv-C-kappa-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFR beta expressing cells
- Title
- Bispecific anti-mPDGFR beta x cotinine scFv-C-kappa-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFR beta expressing cells
- Authors
- Kim, Soohyun; Kim, Hyori; Jo, Dong Hyun; Kim, Jeong Hun; Kim, Su Ree; Kang, Dongmin; Hwang, Dobeen; Chung, Junho
- Ewha Authors
- 강동민
- SCOPUS Author ID
- 강동민
- Issue Date
- 2019
- Journal Title
- METHODS
- ISSN
- 1046-2023
1095-9130
- Citation
- METHODS vol. 154, pp. 125 - 135
- Keywords
- Antibody-drug conjugate; Bispecific antibody; Cotinine; Duocarmycin
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Antibody selection for antibody-drug conjugates (ADCs) has traditionally depended on its internalization into the target cell, although ADC efficacy also relies on recycling of the receptor-ADC complex, endo-lysosomal trafficking, and subsequent linker/antibody proteolysis. In this study, we observed that a bispecific anti-murine platelet-derived growth factor receptor beta (mPDGFR beta) x cotinine single-chain variable fragment (scFv)-kappa constant region (C-kappa)-scFv fusion protein and cotinine-duocarmycin can form an ADC-like complex to induce cytotoxicity against rnPDGFR beta expressing cells. Multiple anti-mPDGFR beta antibody candidates can be produced in this bispecific scFv-C-kappa-scFv fusion protein format and tested for their ability to deliver cotinine-conjugated cytotoxic drugs, thus providing an improved approach for antibody selection in ADC development.
- DOI
- 10.1016/j.ymeth.2018.10.002
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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