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SPSB1 enhances ovarian cancer cell survival by destabilizing p21

Title
SPSB1 enhances ovarian cancer cell survival by destabilizing p21
Authors
Kim, Hyun-JungKim, Hye JinKim, Mi-KyungBae, Moon KyoungSung, Hye YounAhn, Jung-HyuckKim, Yun HwanKim, Seung CheolJu, Woong
Ewha Authors
김승철주웅안정혁성혜윤김윤환김현정김미경
SCOPUS Author ID
김승철scopus; 주웅scopus; 안정혁scopus; 성혜윤scopus; 김윤환scopus; 김현정scopus
Issue Date
2019
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
0006-291XJCR Link

1090-2104JCR Link
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 510, no. 3, pp. 364 - 369
Keywords
SPSB1Ovarian cancerp21 destabilizationUbiquitinationCell survival
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells. (C) 2019 Elsevier Inc. All rights reserved.
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DOI
10.1016/j.bbrc.2019.01.088
Appears in Collections:
의과대학 > 의학과 > Journal papers
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