View : 477 Download: 0

Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation

Title
Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation
Authors
Lim H.B.Lee J.H.Lee K.-J.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopusscopus
Issue Date
1995
Journal Title
Electrophoresis
ISSN
0173-0835JCR Link
Citation
Electrophoresis vol. 16, no. 4, pp. 674 - 678
Indexed
SCI; SCIE; SCOPUS scopus
Document Type
Conference Paper
Abstract
A low-cost and highly sensitive laser-induced fluorescence detector for on-column detection systems was constructed and its applications were demonstrated in protein separation by capillary electrophoresis. The limit of detection (LOD) at a signal-to-noise ratio of 2 for Lissamine 20 was about 1.4 x 10 -21 mole at 10 nL sample injection and the relative standard deviation (RSD) for 1.5 x 10 -11 M of Lissamine 20 solution was about 15%. These values are comparable to or even better than those reported using a similar on-column detection system. This sensitive LIF detection was applied for the study of tryptic digestion of insulin and for urinary protein profiling.
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE