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Application of capillary electrophoresis to amino acid sequencing of peptide

Title
Application of capillary electrophoresis to amino acid sequencing of peptide
Authors
Kim N.J.Kim J.H.Lee K.-J.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopus
Issue Date
1995
Journal Title
Electrophoresis
ISSN
0173-0835JCR Link
Citation
Electrophoresis vol. 16, no. 4, pp. 510 - 515
Indexed
SCI; SCIE; SCOPUS scopus
Document Type
Conference Paper
Abstract
Separations of twenty phenylthiohydantoin (PTH) amino acids, and amino acid derivatives, resulting from the Edman degradation of peptides and proteins, were optimized for peptide sequencing by capillary electrophoresis. Manual sequencing of angiotensin II was performed by Edman degradation and capillary electrophoresis of the PTH amino acid obtained after each cycle. The results were compared with those of an automated conventional protein sequencer. Interfacing capillary electrophoresis with Edman degradation provides an additional option for protein sequencing.
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