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dc.contributor.author박시재-
dc.date.accessioned2019-01-02T16:30:14Z-
dc.date.available2019-01-02T16:30:14Z-
dc.date.issued2018-
dc.identifier.issn2073-4344-
dc.identifier.otherOAK-24033-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/248066-
dc.description.abstractCorynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (P-vgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of P-vgb (P-vgb, P-vgb4, and P-vgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of P-vgb increased from single to eight (P-vgb8) repeats. Furthermore, we demonstrated the application of the new P-vgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadB(mut). We observed that 5-AVA and GABA production by the recombinant strains increased as the number of P-vgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of P-vgb8 (3.69 +/- 0.07 g/L) than the one under the control of P-vgb (3.43 +/- 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of P-vgb used for GadB(mut) expression increased from single (4.81-5.31 g/L) to eight repeats (4.94-5.58 g/L).-
dc.languageEnglish-
dc.publisherMDPI-
dc.subjectCorynebacterium glutamicum-
dc.subjectP-vgb-
dc.subjecttunable expression system-
dc.subjectexpression vectors-
dc.subjectsynthetic biology-
dc.subjectVitreoscilla-
dc.subjectvgb-
dc.titleConstruction of a Vitreoscilla Hemoglobin Promoter-Based Tunable Expression System for Corynebacterium glutamicum-
dc.typeArticle-
dc.relation.issue11-
dc.relation.volume8-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.journaltitleCATALYSTS-
dc.identifier.doi10.3390/catal8110561-
dc.identifier.wosidWOS:000451150200077-
dc.author.googleBaritugo, Kei-Anne-
dc.author.googleKim, Hee Taek-
dc.author.googleRhie, Mi Na-
dc.author.googleJo, Seo Young-
dc.author.googleKhang, Tae Uk-
dc.author.googleKang, Kyoung Hee-
dc.author.googleSong, Bong Keun-
dc.author.googleLee, Binna-
dc.author.googleSong, Jae Jun-
dc.author.googleChoi, Jong Hyun-
dc.author.googleLee, Dae-Hee-
dc.author.googleJoo, Jeong Chan-
dc.author.googlePark, Si Jae-
dc.contributor.scopusid박시재(57191670770)-
dc.date.modifydate20210929141221-
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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