Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이혁진 | * |
dc.date.accessioned | 2018-12-07T16:30:40Z | - |
dc.date.available | 2018-12-07T16:30:40Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1543-8384 | * |
dc.identifier.other | OAK-20676 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247391 | - |
dc.description.abstract | Biological ligands such as aptamer, antibody, glucose, and peptide have been widely used to bind specific surface molecules or receptors in tumor cells or subcellular structures to improve tumor-targeting efficiency of nanoparticles. However, this active-targeting strategy has limitations for tumor targeting due to inter- and intraheterogeneity of tumors. In this study, we demonstrated an alternative active-targeting strategy using metabolic engineering and bioorthogonal click reaction to improve tumor-targeting efficiency of nanoparticles. We observed that azide-containing chemical reporters were successfully generated onto surface glycans of various tumor cells such as lung cancer (A549), brain cancer (U87), and breast cancer (BT-474, MDA-MB231, MCF-7) via metabolic engineering in vitro. In addition, we compared tumor targeting of artificial azide reporter with bicyclononyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-CNPs) and integrin αvβ3 with cyclic RGD-conjugated CNPs (cRGD-CNPs) in vitro and in vivo. Fluorescence intensity of azide-reporter-targeted BCN-CNPs in tumor tissues was 1.6-fold higher and with a more uniform distribution compared to that of cRGD-CNPs. Moreover, even in the isolated heterogeneous U87 cells, BCN-CNPs could bind artificial azide reporters on tumor cells more uniformly (∼92.9%) compared to cRGD-CNPs. Therefore, the artificial azide-reporter-targeting strategy can be utilized for targeting heterogeneous tumor cells via bioorthogonal click reaction and may provide an alternative method of tumor targeting for further investigation in cancer therapy. © 2017 American Chemical Society. | * |
dc.description.sponsorship | Korea Institute of Science and Technology | * |
dc.language | English | * |
dc.publisher | American Chemical Society | * |
dc.subject | active tumor targeting | * |
dc.subject | bioorthogonal click reaction | * |
dc.subject | heterogeneity | * |
dc.subject | metabolic glycoengineering | * |
dc.title | Artificial Chemical Reporter Targeting Strategy Using Bioorthogonal Click Reaction for Improving Active-Targeting Efficiency of Tumor | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1558 | * |
dc.relation.lastpage | 1570 | * |
dc.relation.journaltitle | Molecular Pharmaceutics | * |
dc.identifier.doi | 10.1021/acs.molpharmaceut.6b01083 | * |
dc.identifier.wosid | WOS:000400633300023 | * |
dc.identifier.scopusid | 2-s2.0-85018370929 | * |
dc.author.google | Yoon H.Y. | * |
dc.author.google | Shin M.L. | * |
dc.author.google | Shim M.K. | * |
dc.author.google | Lee S. | * |
dc.author.google | Na J.H. | * |
dc.author.google | Koo H. | * |
dc.author.google | Lee H. | * |
dc.author.google | Kim J.-H. | * |
dc.author.google | Lee K.Y. | * |
dc.author.google | Kim K. | * |
dc.author.google | Kwon I.C. | * |
dc.contributor.scopusid | 이혁진(55233457200) | * |
dc.date.modifydate | 20240220111730 | * |