Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이공주 | - |
dc.date.accessioned | 2018-12-07T16:30:28Z | - |
dc.date.available | 2018-12-07T16:30:28Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-21223 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247324 | - |
dc.description.abstract | When cells are exposed to heat shock and various other stresses, heat shock factor 1 (HSF1) is activated, and the heat shock response (HSR) is elicited. To better understand the molecular regulation of the HSR, we used 2D-PAGE– based proteome analysis to screen for heat shock–induced post-translationally modified cellular proteins. Our analysis revealed that two protein spots typically present on 2D-PAGE gels and containing heterogeneous nuclear ribonucleoprotein K (hnRNP K) with trioxidized Cys132 disappeared after the heat shock treatment and reappeared during recovery, but the total amount of hnRNP K protein remained unchanged. We next tested whether hnRNP K plays a role in HSR by regulating HSF1 and found that hnRNP K inhibits HSF1 activity, resulting in reduced expression of hsp70 and hsp27 mRNAs. hnRNP K also reduced binding affinity of HSF1 to the heat shock element by directly interacting with HSF1 but did not affect HSF1 phosphorylation– dependent activation or nuclear localization. hnRNP K lost its ability to induce these effects when its Cys132 was substituted with Ser, Asp, or Glu. These findings suggest that hnRNP K inhibits transcriptional activity of HSF1 by inhibiting its binding to heat shock element and that the oxidation status of Cys132 in hnRNP K is critical for this inhibition. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.description.sponsorship | Seoul National University | - |
dc.language | English | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Heterogeneous nuclear ribonucleoprotein K inhibits heat shock-induced transcriptional activity of heat shock factor 1 | - |
dc.type | Article | - |
dc.relation.issue | 31 | - |
dc.relation.volume | 292 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 12801 | - |
dc.relation.lastpage | 12812 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M117.774992 | - |
dc.identifier.wosid | WOS:000407220100012 | - |
dc.identifier.scopusid | 2-s2.0-85026766979 | - |
dc.author.google | Kim H.-J. | - |
dc.author.google | Lee J.-J. | - |
dc.author.google | Cho J.-H. | - |
dc.author.google | Jeong J. | - |
dc.author.google | Park A.Y. | - |
dc.author.google | Kang W. | - |
dc.author.google | Lee K.-J. | - |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | - |
dc.date.modifydate | 20230208115507 | - |