View : 238 Download: 0
A metagenomic analysis provides a culture-independent pathogen detection for atopic dermatitis
- A metagenomic analysis provides a culture-independent pathogen detection for atopic dermatitis
- Kim M.-H.; Rho M.; Choi J.-P.; Choi H.-I.; Park H.-K.; Song W.-J.; Min T.-K.; Cho S.-H.; Cho Y.-J.; Kim Y.-K.; Yang S.; Pyun B.Y.
- Ewha Authors
- 조영주; 김민혜
- SCOPUS Author ID
- 조영주; 김민혜
- Issue Date
- Journal Title
- Allergy, Asthma and Immunology Research
- Allergy, Asthma and Immunology Research vol. 9, no. 5, pp. 453 - 461
- Atopic dermatitis; Extracellular vesicle; Metagenomic analysis
- Korean Academy of Asthma, Allergy and Clinical Immunology
- SCIE; SCOPUS; KCI
- Document Type
Show the fulltext
- Purpose: Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested a successive identification of AD-associated microbes, followed by a culture-independent serum detection of the identified microbe. Methods: A total of 43 genomic DNA preparations from washing fluid of the cubital fossa of 6 healthy controls, skin lesions of 27 AD patients, 10 of which later received treatment (post-treatment), were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform. Results: Microbial diversity was decreased in AD, and was restored following treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, and Streptococcus, whereas Alcaligenaceae (f), Sediminibacterium, and Lactococcus were characteristic of healthy skin. An enzyme-linked immunosorbent assay (ELISA) showed that serum could be used as a source for the detection of Staphylococcus aureus extracellular vesicles (EVs). S. aureus EV-specific immunoglobulin G (IgG) and immunoglobulin E (IgE) were quantified in the serum. Conclusions: A metagenomic analysis together with a serum detection of pathogen-specific EVs provides a model for successive identification and diagnosis of pathogens of AD. © The Korean Academy of Asthma, Allergy and Clinical Immunology.
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.