Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2018-12-07T16:30:22Z | - |
dc.date.available | 2018-12-07T16:30:22Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-21352 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247290 | - |
dc.description.abstract | The syndecan family of heparan sulfate proteoglycans contributes to cell adhesion and communication by serving as co-receptors for cell signaling and extracellular matrix molecules. Syndecan-2 is located at the cell surface, and we previously reported that it induces matrix metalloproteinase-7 (MMP-7) expression in colon cancer cells. However, the underlying regulatory mechanisms are unknown. Here, we report that overexpression of syndecan-2 in HT-29 colon cancer cells increases the phosphorylation of focal adhesion kinase (FAK) and ERK in parallel with up-regulated MMP-7 expression, but a syndecan-2 mutant lacking the cytoplasmic domain showed significant reductions in these effects. Consistent with this observation, FAK inhibition via FAK-related non-kinase expression or inhibition of ERK with the ERK1/2 inhibitor SCH772984 diminished the syndecan-2–mediated up-regulation of MMP-7. Activation of PKC enhanced syndecan-2–mediated MMP-7 expression, whereas inhibition of PKC had the opposite effect. Of note, the exogenous expression of syndecan-2 triggered localization of PKC to the membrane. Expression of syndecan-2 harboring a phosphomimetic (S198E) mutation of the variable region of the cytoplasmic domain enhanced MMP-7 expression and FAK phosphorylation. Finally, experimental suppression of shedding of the syndecan-2 extracellular domain did not significantly affect the syndecan-2–mediated up-regulation of MMP-7 in the early period after syndecan-2 overexpression. Taken together, these findings suggest that syndecan-2’s cytoplasmic domain up-regulates MMP-7 expression in colon cancer cells via PKC-mediated activation of FAK/ERK signaling. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.description.sponsorship | National Research Foundation of Korea | - |
dc.language | English | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Syndecan-2 cytoplasmic domain up-regulates matrix metalloproteinase-7 expression via the protein kinase Cγ–mediated FAK/ERK signaling pathway in colon cancer | - |
dc.type | Article | - |
dc.relation.issue | 39 | - |
dc.relation.volume | 292 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 16321 | - |
dc.relation.lastpage | 16332 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M117.793752 | - |
dc.identifier.wosid | WOS:000412109400030 | - |
dc.identifier.scopusid | 2-s2.0-85030027854 | - |
dc.author.google | Jang B. | - |
dc.author.google | Jung H. | - |
dc.author.google | Choi S. | - |
dc.author.google | Lee Y.H. | - |
dc.author.google | Lee S.-T. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20230201093717 | - |