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IL-10 plays a pivotal role in tamoxifen-induced spasmolytic polypeptide-expressing metaplasia in gastric mucosa
- Title
- IL-10 plays a pivotal role in tamoxifen-induced spasmolytic polypeptide-expressing metaplasia in gastric mucosa
- Authors
- Lee C.; Lee H.; Hwang S.Y.; Moon C.M.; Hong S.N.
- Ewha Authors
- 문창모
- SCOPUS Author ID
- 문창모
- Issue Date
- 2017
- Journal Title
- Gut and Liver
- ISSN
- 1976-2283
- Citation
- Gut and Liver vol. 11, no. 6, pp. 789 - 797
- Keywords
- Interleukin-10; Spasmolytic polypeptide-expressing metaplasia; Stomach neoplasms
- Publisher
- Joe Bok Chung
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Background/Aims: Gastric cancer evolves in the pathologic mucosal milieu, and its development is characterized by both the loss of acid-secreting parietal cells and mucosal cell metaplasia, called spasmolytic polypeptide-expressing metaplasia (SPEM). Cytokines, such as interleukin (IL)-10, IL-1β, and IL-6, play a key role in gastric carcinogenesis. However, changes in the cytokine profile of SPEM have not been evaluated. Methods: To induce SPEM in mouse stomachs, C57BL/6 mice were intraperitoneally injected with tamoxifen and sacrificed at 3, 10, and 21 days after treatment. RNA-sequencing (RNA-seq) and a multiplex bead array were used to measure cytokines in the stomachs of tamoxifen-treated/control mice. Results: The administration of tamoxifen led to the rapid development and histological normalization of SPEM 3 and 10 days after administration, respectively. RNA-seq revealed that the expression of IL-10 was decreased 3 days after tamoxifen administration. The multiplex assay identified a significant decline in IL-10 levels 3 days after tamoxifen treatment (58.38±34.44 pg/mL vs 94.09±4.98 pg/mL, p=0.031), which normalized at 10 and 21 days after tamoxifen treatment. Immunofluorescence staining confirmed that IL-10 expression was markedly decreased at the time of SPEM development and subsequently returned to normal, accompanied by a reversal in histologic changes. Conclusions: IL-10 may play a pivotal role in the tamoxifen-induced acute development of gastric SPEM.
- DOI
- 10.5009/gnl16454
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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