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Kaempferol inhibits enterovirus proliferation through MAPK signal regulation

Title
Kaempferol inhibits enterovirus proliferation through MAPK signal regulation
Authors
Jang J.-H.Jeong H.-I.Lim B.-K.Nam S.-J.
Ewha Authors
남상집
SCOPUS Author ID
남상집scopusscopus
Issue Date
2017
Journal Title
Korean Journal of Pharmacognosy
ISSN
0253-3073JCR Link
Citation
Korean Journal of Pharmacognosy vol. 48, no. 3, pp. 173 - 178
Keywords
Coxsackievirus B3EnterovirusKaempferolMyocarditisPlant extract
Publisher
Korean Society of Pharmacognosy
Indexed
SCOPUS; KCI scopus
Document Type
Article
Abstract
We investigated the efficacy of single compound of plant extract in coxsackievirus B3 (CVB3) infection. CVB3 is a main cause of Hand-foot-mouth diseases (HFMD) and viral myocarditis in children and adult. Several single compounds of plant extract were purified by HPLC and tested as antiviral drug candidate. Among them, kaempferol was selected to effective anti-enterovirus compound by HeLa cells survival assay. CVB3 infected HeLa cells were treated with kaempferol (100 μg/ml- 100 ng/ml) and their antiviral effect was confirmed. After 16 hours of treatment, HeLa cells were lysed and proteins were extracted for western blot analysis. CVB3 viral capsid protein VP1 production and transcription factor eIF4G-1 cleavage was significantly decreased in 100 μg/ml kaempferol treatment. Virus replication was observed by virus RNA amplification. Kaempferol strongly reduced virus positive and negative strand RNA amplification. Moreover, MAPK signal induced by CVB3 infection, pERK and pmTOR, kaempferol treatment significantly inhibited the activity. Plant extract single compound, kaempferol, is a strong candidate to be developed non-toxic anti-enterovirus treatment agent. © 2017, Korean Society of Pharmacognosy. All rights reserved.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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