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Interaction of macrophages with apoptotic cells inhibits transdifferentiation and invasion of lung fibroblasts
- Title
- Interaction of macrophages with apoptotic cells inhibits transdifferentiation and invasion of lung fibroblasts
- Authors
- Kim, Yong-Bae; Yoon, Young-So; Choi, Youn-Hee; Park, Eun-Mi; Kang, Jihee Lee
- Ewha Authors
- 이지희; 박은미; 최윤희; 김용배
- SCOPUS Author ID
- 이지희; 박은미; 최윤희; 김용배
- Issue Date
- 2017
- Journal Title
- ONCOTARGET
- ISSN
- 1949-2553
- Citation
- ONCOTARGET vol. 8, no. 68, pp. 112297 - 112312
- Keywords
- apoptotic cells; macrophages; lung fibroblasts; myofibroblast; invasion
- Publisher
- IMPACT JOURNALS LLC
- Indexed
- SCOPUS
- Document Type
- Article
- Abstract
- The invasion of activated fibroblasts is a key mechanism of tissue fibrosis pathology. The recognition and uptake of apoptotic cells can induce the anti-fibrogenic programming of macrophages. We demonstrate that after interacting with apoptotic cells, macrophages secrete bioactive molecules that antagonize TGF-beta 1-induced increases in myofibroblast (fibroproliferative) phenotypic markers and reduce the enhanced invasive capacity of TGF-beta 1- or EGF-treated mouse lung fibroblasts (MLg). Furthermore, numerous treatment strategies prevented the anti-fibrotic effects of conditioned media, including transfection of macrophages with COX-2 or RhoA siRNAs or treatment of MLg cells with receptor antagonists for prostaglandin E-2 (PGE(2)), PGD(2), or hepatocyte growth factor (HGF). Additionally, administration of apoptotic cells in vivo inhibited the bleomycin-mediated invasive capacity of primary fibroblasts, as well as adhesion and extracellular matrix protein mRNA expression. These data suggest that the anti-fibrogenic programming of macrophages by apoptotic cells can be used as a novel tool to control the progressive fibrotic reaction.
- DOI
- 10.18632/oncotarget.22737
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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