Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2018-11-21T16:30:54Z | - |
dc.date.available | 2018-11-21T16:30:54Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 2041-1723 | * |
dc.identifier.other | OAK-21938 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246903 | - |
dc.description.abstract | Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-Transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a d-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% d-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-d-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of d-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha β-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l-1 of poly(61.9 mol% 3HB-co-38.1 mol% d-phenyllactate). Furthermore, different aromatic polyesters containing d-mandelate and d-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources. © 2017 The Author(s). | * |
dc.language | English | * |
dc.publisher | Nature Publishing Group | * |
dc.title | One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 9 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Nature Communications | * |
dc.identifier.doi | 10.1038/s41467-017-02498-w | * |
dc.identifier.wosid | WOS:000419446600007 | * |
dc.identifier.scopusid | 2-s2.0-85042799027 | * |
dc.author.google | Yang J.E. | * |
dc.author.google | Park S.J. | * |
dc.author.google | Kim W.J. | * |
dc.author.google | Kim H.J. | * |
dc.author.google | Kim B.J. | * |
dc.author.google | Lee H. | * |
dc.author.google | Shin J. | * |
dc.author.google | Lee S.Y. | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |