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A new kind of rhodamine-based fluorescence turn-on probe for monitoring ATP in mitochondria

Title
A new kind of rhodamine-based fluorescence turn-on probe for monitoring ATP in mitochondria
Authors
Liu Y.Lee D.Wu D.Swamy K.M.K.Yoon J.
Ewha Authors
윤주영K.M.K Swamy
SCOPUS Author ID
윤주영scopus
Issue Date
2018
Journal Title
Sensors and Actuators, B: Chemical
ISSN
0925-4005JCR Link
Citation
Sensors and Actuators, B: Chemical vol. 265, pp. 429 - 434
Keywords
ATP sensorColorimetric chemosensorFluorescence sensorMitochondria targetingRhodamine
Publisher
Elsevier B.V.
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
We have developed a new kind of colorimetric and fluorescence “turn-on” probe based on rhodamine derivatives for the detection of adenosine-5′-triphosphate (ATP) through hydrogen bond interactions. Upon the addition of 5 mM ATP, the probe 1 shows a 65-fold enhancement in fluorescence intensity and a color change from colorless to pink. The probe 2 also shows very obvious color and fluorescence change with the presence of ATP. The experimental results show that this kind of probe has high specificity towards ATP over other anions, metal ions, and other nucleoside polyphosphates, except ADP. The enhanced emission and naked eye changes are attributed to spirolactam ring-opening, which is pH independent (pH (4.0–7.4)). The more important finding from our results is that the electron withdrawing group in the chain decreases the binding ability to ATP. The live cell imaging experiments in Hela cells indicated that probe 2 has cell permeability, and mainly locates to mitochondria. © 2018 Elsevier B.V.
DOI
10.1016/j.snb.2018.03.081
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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