View : 228 Download: 52

Full metadata record

DC Field Value Language
dc.contributor.author임재향-
dc.date.accessioned2018-11-15T16:30:16Z-
dc.date.available2018-11-15T16:30:16Z-
dc.date.issued2018-
dc.identifier.issn2045-2322-
dc.identifier.otherOAK-23550-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/246509-
dc.description.abstractRecent studies have indicated that protease-activated receptor-1 (PAR-1) is involved in cytoprotective and anti-inflammatory responses in endothelial cells (ECs). However, the role of PAR-1 in laminar flow-mediated atheroprotective responses remains unknown. Herein, we investigated whether PAR-1 regulates laminar flow-mediated mechanotransduction in ECs. Confocal analysis showed that PAR-1 was internalized into early endosomes in response to laminar flow. In addition, flow cytometry analysis showed that cell surface expression of PAR-1 was reduced by laminar flow, suggesting that PAR-1 was activated in response to laminar flow. Depletion of PAR-1 using human PAR-1 siRNA inhibited unidirectional laminar flow-mediated actin stress fiber formation and cellular alignment as well as atheroprotective gene expressions in HUVECs. Moreover, PAR-1 knockdown inhibited laminar flow-stimulated eNOS phosphorylation, and inhibited the phosphorylations of Src, AMPK, ERK5 and HDAC5. Furthermore, PAR-1 depletion inhibited laminar flow-mediated anti-inflammatory responses as demonstrated by reduced TNF alpha-induced VCAM-1 expression and by monocyte adhesion to HUVECs, and prevented laminar flow-mediated anti-apoptotic response. An investigation of the role of PAR-1 in vasomotor modulation using mouse aortic rings revealed that acetylcholine-induced vasorelaxation was diminished in PAR-1 deficient mice compared to littermate controls. Taken together, these findings suggest that PAR-1 be viewed as a novel pharmacologic target for the treatment of vascular diseases, including atherosclerosis.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titlePAR-1 is a novel mechano-sensor transducing laminar flow-mediated endothelial signaling-
dc.typeArticle-
dc.relation.volume8-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.journaltitleSCIENTIFIC REPORTS-
dc.identifier.doi10.1038/s41598-018-33222-3-
dc.identifier.wosidWOS:000447083100063-
dc.identifier.scopusid2-s2.0-85054777778-
dc.author.googleKim, Suji-
dc.author.googleHan, Jung-Hwa-
dc.author.googleNam, Dae-Hwan-
dc.author.googleKim, Geun-Young-
dc.author.googleLim, Jae Hyang-
dc.author.googleKim, Jae-Ryong-
dc.author.googleWoo, Chang-Hoon-
dc.contributor.scopusid임재향(7403454262)-
dc.date.modifydate20190901081003-


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE