Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임재향 | * |
dc.date.accessioned | 2018-11-15T16:30:16Z | - |
dc.date.available | 2018-11-15T16:30:16Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 2045-2322 | * |
dc.identifier.other | OAK-23550 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246509 | - |
dc.description.abstract | Recent studies have indicated that protease-activated receptor-1 (PAR-1) is involved in cytoprotective and anti-inflammatory responses in endothelial cells (ECs). However, the role of PAR-1 in laminar flow-mediated atheroprotective responses remains unknown. Herein, we investigated whether PAR-1 regulates laminar flow-mediated mechanotransduction in ECs. Confocal analysis showed that PAR-1 was internalized into early endosomes in response to laminar flow. In addition, flow cytometry analysis showed that cell surface expression of PAR-1 was reduced by laminar flow, suggesting that PAR-1 was activated in response to laminar flow. Depletion of PAR-1 using human PAR-1 siRNA inhibited unidirectional laminar flow-mediated actin stress fiber formation and cellular alignment as well as atheroprotective gene expressions in HUVECs. Moreover, PAR-1 knockdown inhibited laminar flow-stimulated eNOS phosphorylation, and inhibited the phosphorylations of Src, AMPK, ERK5 and HDAC5. Furthermore, PAR-1 depletion inhibited laminar flow-mediated anti-inflammatory responses as demonstrated by reduced TNF alpha-induced VCAM-1 expression and by monocyte adhesion to HUVECs, and prevented laminar flow-mediated anti-apoptotic response. An investigation of the role of PAR-1 in vasomotor modulation using mouse aortic rings revealed that acetylcholine-induced vasorelaxation was diminished in PAR-1 deficient mice compared to littermate controls. Taken together, these findings suggest that PAR-1 be viewed as a novel pharmacologic target for the treatment of vascular diseases, including atherosclerosis. | * |
dc.language | English | * |
dc.publisher | NATURE PUBLISHING GROUP | * |
dc.title | PAR-1 is a novel mechano-sensor transducing laminar flow-mediated endothelial signaling | * |
dc.type | Article | * |
dc.relation.volume | 8 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | SCIENTIFIC REPORTS | * |
dc.identifier.doi | 10.1038/s41598-018-33222-3 | * |
dc.identifier.wosid | WOS:000447083100063 | * |
dc.identifier.scopusid | 2-s2.0-85054777778 | * |
dc.author.google | Kim, Suji | * |
dc.author.google | Han, Jung-Hwa | * |
dc.author.google | Nam, Dae-Hwan | * |
dc.author.google | Kim, Geun-Young | * |
dc.author.google | Lim, Jae Hyang | * |
dc.author.google | Kim, Jae-Ryong | * |
dc.author.google | Woo, Chang-Hoon | * |
dc.contributor.scopusid | 임재향(7403454262) | * |
dc.date.modifydate | 20240220101019 | * |