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Separation of gangliosides using cyclodextrin in capillary zone electrophoresis

Title
Separation of gangliosides using cyclodextrin in capillary zone electrophoresis
Authors
Young S.Y.Young S.K.Jhon G.-J.Park J.
Ewha Authors
전길자
SCOPUS Author ID
전길자scopus
Issue Date
1993
Journal Title
Journal of Chromatography A
ISSN
0021-9673JCR Link
Citation
Journal of Chromatography A vol. 652, no. 2, pp. 431 - 439
Indexed
SCIE; SCOPUS scopus
Document Type
Article
Abstract
Gangliosides are glycosphingolipids containing sialic acid. These glycolipids have been suggested to play important roles in biological processes such as cell growth differentiation and malignant transformation. Based on these proposed biological functions, gangliosides can be used as diagnostic tools and therapeutics for various human diseases. In this study, capillary zone electrophoresis (CZE) was used to determine the major gangliosides GM1, GD1a, GD1b and GT1b in mammalian brains, in addition to GM3 and Lac. Enhancement of selectivity and efficiency of separation was obtained by using 50 mM borate-phosphate buffer containing 16.5 mM α-cyclodextrin (α-CD). Under this condition, several forms of gangliosides were successfully separated from extracts of deer antler, apricot seed and rat brain. The results demonstrate that the CD-modified CZE is a useful method for detecting glycolipids from various biological matrices. © 1993.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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