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Changes in Intracellular Ca2+ Concentration of Rabbit Coronary Artery Smooth Muscle Cell during Ischemic Cardioplegic Period
- Title
- Changes in Intracellular Ca2+ Concentration of Rabbit Coronary Artery Smooth Muscle Cell during Ischemic Cardioplegic Period
- Authors
- Lee Y.H.; Choi G.B.; Ahn D.S.; Kang B.S.
- Ewha Authors
- 최규복
- SCOPUS Author ID
- 최규복
- Issue Date
- 1996
- Journal Title
- Yonsei Medical Journal
- ISSN
- 0513-5796
- Citation
- Yonsei Medical Journal vol. 37, no. 4, pp. 251 - 261
- Indexed
- SCI; SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- To elucidate the possibility whether an elevation of intracellular Ca2+ concentration ([Ca2+]) in rabbit coronary artery myocytes during ischemic cardioplegic period may serve as one of the mechanisms of the 'no-reflow' phenomenon or not, the changes in [Ca2+]i were measured under ischemic cardioplegia conditions using a fluorescent Ca2+ indicator, fura 2/AM. When single cells were perfused with cardioplegic or ischemic cardioplegic solutions, [Ca2+]i was significantly increased and the degree of [Ca2+]i elevation was further augmented by the ischemic cardioplegic solution. Pretreatment of a sarcoplasmic reticulum emptying agent, 20 mM caffeine, had no effect on ischemic cardioplegia-induced [Ca2+]i changes, but application of a Ca2+ channel blocker, 5 × 10-7M nifedipine, or an antagonist of Na+/Ca2+ exchange, 5 mM Ni2+, significantly inhibited the [Ca2+]i elevation, respectively. The magnitude of ischemic cardioplegia-induced [Ca2+]i elevation was dependent on the Ca2+ concentration of perfusate in the range of 0 and 2.5 mM. When Ni2+ was added to the reperfusion solution, recovery of ischemic cardioplegia-induced [Ca2+]i elevation was very rapid compared with the controls. It is concluded that ischemic cardioplegia-induced [Ca2+]i elevation may serve as one of the mechanisms of the 'no-reflow' phenomenon in rabbit coronary artery smooth muscle, cells. We propose that Na+/Ca2+ exchange may serve as a key function in ischemic cardiopelgia-induced [Ca2+]i elevation.
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- 의과대학 > 의학과 > Journal papers
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