Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전길자 | - |
dc.contributor.author | 이민영 | - |
dc.date.accessioned | 2018-06-02T08:15:06Z | - |
dc.date.available | 2018-06-02T08:15:06Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.other | OAK-17048 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/244396 | - |
dc.description.abstract | Interactions between ganglioside GM3 and glucose transporter, GLUT1 were studied by measuring the effect of GM3 on steady-state and time-resolved fluorescence of purified GLUT1 in synthetic lipids and on the 3-O-methylglucose uptake by human erythrocytes. The intrinsic tryptophan fluorescence showed a GLUT 1 emission maximum of 335 nm, and increased in the presence of GM3 by 12% without shifting the emission maximum. The fluorescence lifetimes of intrinsic tryptophan on GLUT1 consisted of a long component of 7.8 ns and a short component of 2.3 ns and GM3 increased both lifetime components. Lifetime components were quenched by acrylamide and KI. Acrylamide-induced quenching of long-lifetime components was partly recovered by GM3. However, KI-induced quenching of short- and long-lifetime components was not rescued by GM3. The anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH)-probed dimyristoylphosphatidylcholine (DMPC) model membrane was also increased with GM3 incorporation. The transport rate of 3-O-methylglucose increased by 20% with GM3 incorporation on the erythrocytes. Therefore, GM3 altered the environment of lipid membrane and induced the conformational change of GLUT1. | - |
dc.language | English | - |
dc.title | Effect of ganglioside GM3 on the erythrocyte glucose transporter (GLUT1): Conformational changes measured by steady-state and time-resolved fluorescence spectroscopy | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 30 | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 240 | - |
dc.relation.lastpage | 245 | - |
dc.relation.journaltitle | Journal of Biochemistry and Molecular Biology | - |
dc.identifier.scopusid | 2-s2.0-0031500621 | - |
dc.author.google | Yoon H. | - |
dc.author.google | Lee M. | - |
dc.author.google | Jhon G.-J. | - |
dc.contributor.scopusid | 전길자(6701488476) | - |
dc.contributor.scopusid | 이민영(55582235800) | - |
dc.date.modifydate | 20190901081003 | - |