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Rapid Purification and Characterization of Nucleoside Diphosphate Kinase Isoforms Using ATP-Sepharose Affinity Column Chromatography

Title
Rapid Purification and Characterization of Nucleoside Diphosphate Kinase Isoforms Using ATP-Sepharose Affinity Column Chromatography
Authors
Kim S.Y.Chang K.H.Doh H.-J.Jung J.A.Kim E.Sim C.J.Lee K.-J.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopusscopus
Issue Date
1997
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
Molecules and Cells vol. 7, no. 5, pp. 630 - 634
Indexed
SCI; SCIE; SCOPUS; KCI scopus
Document Type
Article
Abstract
Nucleoside diphosphate kinases (NDP kinases), products of the nm23 gene, catalyze the transfer of the terminal phosphate group of the nucleoside triphosphate to the corresponding diphosphate and may be involved in tumor metastasis suppression, development, and signal transduction. NDP kinases from various sources including human erythrocytes, rat brain tissue and E. coli strain BL21 transformed with pET3C expression plasmids containing nm23-H1 or nm23-H2, were purified in one step to homogeneity using ATP-sepharose affinity column chromatography. This method was applicable for the purification of various NDP kinases which show the same enzymatic activity and immunodetection, but have various molecular weight and quaternary structures.
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약학대학 > 약학과 > Journal papers
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