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Rapid detection of serum HCV RNA by combining reverse transcription and PCR without RNA extraction

Title
Rapid detection of serum HCV RNA by combining reverse transcription and PCR without RNA extraction
Authors
Jang J.S.Lee K.-J.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopusscopus
Issue Date
1996
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
Archives of Pharmacal Research vol. 19, no. 6, pp. 486 - 489
Indexed
SCIE; SCOPUS; KCI scopus
Document Type
Article
Abstract
A simple, rapid, specific and sensitive method for the detection of serum hepatitis C virus (HCV) RNA using the reverse transcription-polymerase chain reaction (RT-PCR) technique without conventional RNA extraction was developed. HCV template RNA from serum was obtained by boiling the serum at 95°C for 2 min, cooling rapidly in ice and removing the proteins by cetrifugation. RT-PCR amplifications including the reverse transcription and first PCR amplification were performed in one vessel containing both of reverse transcriptase and Taq DNA polymerase. The detection of HCV RNA from 10-3 μl serum was possible with this method. The suitability of this method for clinical analysis was evaluated by assaying HCV RNA in 225 patient samples including anti-HCV antibody negatives (13 samples) and positives (212 samples) by enzyme-linked immunosorbent assay test (ELISA). Detections of HCV RNA with this method were in 4 of 13 anti-HCV antibody negative samples (30.8%) and 95 of 212 positive samples (44.8%). The present method can be completed in 1 hr and has a wide range of application for the clinical utilities to determine the viral RNAs.
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약학대학 > 약학과 > Journal papers
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