Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최원자 | - |
dc.date.accessioned | 2018-06-02T08:14:21Z | - |
dc.date.available | 2018-06-02T08:14:21Z | - |
dc.date.issued | 1996 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.other | OAK-17596 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/244093 | - |
dc.description.abstract | By both in vitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene (PGK) promoter DNA and insertion of the leu2-d gene, we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine (G) to adenine (A) change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6∼7 times elevated expression of the adjacent eukaryotic gene, and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene. When expression of the AIDS virus HIV1-gagP17 gene in a lacZ fusion form was examined with this new vector, a 15 times higher level of expression than that from the original PGK promoter was observed. Northern and Southern analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA. Thus, the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast. | - |
dc.language | English | - |
dc.title | Construction of yeast vectors potentially useful for expression of eukaryotic genes as β-galactosidase fusion proteins | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 29 | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 359 | - |
dc.relation.lastpage | 364 | - |
dc.relation.journaltitle | Journal of Biochemistry and Molecular Biology | - |
dc.identifier.scopusid | 2-s2.0-3042944706 | - |
dc.author.google | Chung K.-S. | - |
dc.author.google | Choi W.-J. | - |
dc.author.google | Lee H.-W. | - |
dc.author.google | Kim K.-W. | - |
dc.author.google | Yoo H.-S. | - |
dc.contributor.scopusid | 최원자(7402515987) | - |
dc.date.modifydate | 20230210130537 | - |