Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김화정 | * |
dc.contributor.author | 최신규 | * |
dc.date.accessioned | 2018-05-30T08:14:01Z | - |
dc.date.available | 2018-05-30T08:14:01Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-3297 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243474 | - |
dc.description.abstract | Gq/11 protein-coupled muscarinic receptors are known to regulate the release of soluble amyloid precursor protein (sAPPα) produced by α-secretase processing; however, their signaling mechanisms remain to be elucidated. It has been reported that a muscarinic agonist activates nuclear factor (NF)-κB, a transcription factor that has been shown to play an important role in the Alzheimer disease brain, and that NF-κB activation is regulated by intracellular Ca2+ level. In the present study,weinvestigated whether NF-κB activation plays a role in muscarinic receptor-mediated sAPPα release enhancement and contributes to a changed capacitative Ca2+ entry (CCE), which was suggested to be involved in the muscarinic receptor-mediated stimulation of sAPPα release. Muscarinic receptor-mediated NF-κB activation was confirmed by observing the translocation of the active subunit (p65) of NF-κB to the nucleus by the muscarinic agonist, oxotremorine M (oxoM), in SH-SY5Y neuroblastoma cells expressing muscarinic receptors that are predominantly of the M3 subtype. NF-κB activation and sAPPα release enhancement induced by oxoM were inhibited by NF-κB inhibitors, such as an NF-κB peptide inhibitor (SN50), an IκBα kinase inhibitor (BAY11-7085), a proteasome inhibitor (MG132), the inhibitor of proteasome activity and IκB phosphorylation, pyrrolidine dithiocarbamate, the novel NF-κB activation inhibitor (6-amino-4-(4-phenoxyphenylethylamino) quinazoline), and by an intracellular Ca2+ chelator (TMB-8). Furthermore, both oxoM-induced NF-κB activation and sAPPα release were antagonized by CCE inhibitors (gadolinium or SKF96365) but not by voltage-gated Ca 2+-channel blockers. On the other hand, treatment of cells with NF-κB inhibitors (SN50, BAY11-7085, MG132, or pyrrolidine dithiocarbamate) did not inhibit muscarinic receptor-mediated CCE. These findings provide evidence for the involvement of NF-κB regulated by CCE in muscarinic receptor-mediated sAPPα release enhancement. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | * |
dc.language | English | * |
dc.title | Nuclear factor-κB activated by capacitative Ca2+ entry enhances muscarinic receptor-mediated soluble amyloid precursor protein (sAPPα) release in SH-SY5Y cells | * |
dc.type | Article | * |
dc.relation.issue | 18 | * |
dc.relation.volume | 281 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 12722 | * |
dc.relation.lastpage | 12728 | * |
dc.relation.journaltitle | Journal of Biological Chemistry | * |
dc.identifier.doi | 10.1074/jbc.M601018200 | * |
dc.identifier.wosid | WOS:000237134700064 | * |
dc.identifier.scopusid | 2-s2.0-33744950174 | * |
dc.author.google | Choi S. | * |
dc.author.google | Jin H.K. | * |
dc.author.google | Roh E.-J. | * |
dc.author.google | Ko M.-J. | * |
dc.author.google | Jung J.-E. | * |
dc.author.google | Kim H.-J. | * |
dc.contributor.scopusid | 김화정(56670336100) | * |
dc.date.modifydate | 20240118124308 | * |