Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박은미 | * |
dc.date.accessioned | 2018-05-30T08:13:52Z | - |
dc.date.available | 2018-05-30T08:13:52Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0304-3940 | * |
dc.identifier.other | OAK-3402 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243416 | - |
dc.description.abstract | Activation of cAMP response element binding protein (CREB) is implicated in neuronal survival. The mitogen-activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) activates a transcription factor CREB. Previously, we reported that N-acetyl-O-methyldopamine (NAMDA) protects neurons from ischemia via enhancing ERK dependent CREB phosphorylation. To investigate whether NAMDA induces endogenous survival pathways in apoptotic conditions and whether the neuroprotectant enhances a preexisting survival pathway, we determined the degree of ERK-CREB activation and resistance to apoptosis in staurosporine-treated SK-N-BE(2)C neurons. Compared to forskolin-treated apoptotic cultures, NAMDA-treated cultures induced a minimum activation on ERK (pERK) or CREB (pCREB). However, NAMDA enhanced the activation of ERK and CREB in the presence of forskolin (1.7-fold increase for pCREB, 2.1-fold increase for pERK2, p < 0.05 from forskolin). The effect was completely blocked by a specific MEK inhibitor U0126, suggesting the involvement of ERK dependent CREB signaling. Cleavage of caspase-3 and poly-(ADP-ribose)-polymerase was additively reduced in cultures treated with NAMDA and forskolin simultaneously, but not in the presence of U0126. The data showed that NAMDA enhances forskolin-induced ERK-CREB activation and potentiates forskolin-induced resistance to apoptosis. The study indicates that enhancing endogenous survival pathways by NAMDA combined with other neuroprotective measure(s) might be a useful strategy to reduce apoptosis. © 2006 Elsevier Ireland Ltd. All rights reserved. | * |
dc.language | English | * |
dc.title | Enhanced ERK dependent CREB activation reduces apoptosis in staurosporine-treated human neuroblastoma SK-N-BE(2)C cells | * |
dc.type | Article | * |
dc.relation.issue | 1-2 | * |
dc.relation.volume | 402 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 190 | * |
dc.relation.lastpage | 194 | * |
dc.relation.journaltitle | Neuroscience Letters | * |
dc.identifier.doi | 10.1016/j.neulet.2006.04.004 | * |
dc.identifier.wosid | WOS:000238434300040 | * |
dc.identifier.scopusid | 2-s2.0-33646789490 | * |
dc.author.google | Park E.-M. | * |
dc.author.google | Cho S. | * |
dc.contributor.scopusid | 박은미(35933416400) | * |
dc.date.modifydate | 20240123095000 | * |