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Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection

Title
Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection
Authors
Um S.Y.Jung S.H.Jung S.J.Kim J.I.Chung S.Y.Lee H.J.Han S.B.Choi S.O.
Ewha Authors
이화정
SCOPUS Author ID
이화정scopus
Issue Date
2006
Journal Title
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085JCR Link
Citation
Journal of Pharmaceutical and Biomedical Analysis vol. 41, no. 4, pp. 1458 - 1462
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (λ ex = 305 nm; λ em = 390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL -1 in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL -1. This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin. © 2006.
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DOI
10.1016/j.jpba.2006.03.021
Appears in Collections:
약학대학 > 약학과 > Journal papers
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