View : 408 Download: 0

Full metadata record

DC Field Value Language
dc.contributor.author박진병-
dc.date.accessioned2018-04-25T08:13:37Z-
dc.date.available2018-04-25T08:13:37Z-
dc.date.issued2018-
dc.identifier.issn1359-5113-
dc.identifier.issn1873-3298-
dc.identifier.otherOAK-21913-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/242557-
dc.description.abstractThe conversion of ricinoleic acid from renewable sources to long-chain am-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of Corynebacterium glutamicum as a new microbial strategy for the biotransformation of fatty acids. The established strain Escherichia coli failed to grow at 5 mM n-heptanoic acid, while the specific growth rate of C. glutamicum declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in C. glutamicum, and confirmed that the C. glutamicum biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant C. glutamicum whole-cell bioconversion. At a cultivation temperature of 30 degrees C and a reaction temperature of 35 degrees C, and in the presence of 0.09 g/L Triton X-100, the whole-cell C. glutamicum biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant C. glutamicum-based biocatalyst.-
dc.languageEnglish-
dc.publisherELSEVIER SCI LTD-
dc.subjectBiotransformation-
dc.subjectCorynebacterium glutamicum-
dc.subjectUndec-9-enoic acid-
dc.subjectHeptyl ester-
dc.subjectRenewable fatty acid-
dc.titleMicrobial synthesis of undec-9-enoic acid, heptyl ester from renewable fatty acids using recombinant Corynebacterium glutamicum-based whole-cell biocatalyst-
dc.typeArticle-
dc.relation.volume66-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage61-
dc.relation.lastpage69-
dc.relation.journaltitlePROCESS BIOCHEMISTRY-
dc.identifier.doi10.1016/j.procbio.2017.12.009-
dc.identifier.wosidWOS:000427668800006-
dc.identifier.scopusid2-s2.0-85039805553-
dc.author.googleKim, Hyeonsoo-
dc.author.googleYang, Jeongmo-
dc.author.googleCho, Sukhyeong-
dc.author.googleJeong, Kijun-
dc.author.googlePark, Jinbyung-
dc.author.googleLee, Jinwon-
dc.contributor.scopusid박진병(15036390700)-
dc.date.modifydate20210915134756-
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE