Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박진병 | * |
dc.date.accessioned | 2018-04-25T08:13:37Z | - |
dc.date.available | 2018-04-25T08:13:37Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 1359-5113 | * |
dc.identifier.issn | 1873-3298 | * |
dc.identifier.other | OAK-21913 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242557 | - |
dc.description.abstract | The conversion of ricinoleic acid from renewable sources to long-chain am-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of Corynebacterium glutamicum as a new microbial strategy for the biotransformation of fatty acids. The established strain Escherichia coli failed to grow at 5 mM n-heptanoic acid, while the specific growth rate of C. glutamicum declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in C. glutamicum, and confirmed that the C. glutamicum biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant C. glutamicum whole-cell bioconversion. At a cultivation temperature of 30 degrees C and a reaction temperature of 35 degrees C, and in the presence of 0.09 g/L Triton X-100, the whole-cell C. glutamicum biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant C. glutamicum-based biocatalyst. | * |
dc.language | English | * |
dc.publisher | ELSEVIER SCI LTD | * |
dc.subject | Biotransformation | * |
dc.subject | Corynebacterium glutamicum | * |
dc.subject | Undec-9-enoic acid | * |
dc.subject | Heptyl ester | * |
dc.subject | Renewable fatty acid | * |
dc.title | Microbial synthesis of undec-9-enoic acid, heptyl ester from renewable fatty acids using recombinant Corynebacterium glutamicum-based whole-cell biocatalyst | * |
dc.type | Article | * |
dc.relation.volume | 66 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 61 | * |
dc.relation.lastpage | 69 | * |
dc.relation.journaltitle | PROCESS BIOCHEMISTRY | * |
dc.identifier.doi | 10.1016/j.procbio.2017.12.009 | * |
dc.identifier.wosid | WOS:000427668800006 | * |
dc.identifier.scopusid | 2-s2.0-85039805553 | * |
dc.author.google | Kim, Hyeonsoo | * |
dc.author.google | Yang, Jeongmo | * |
dc.author.google | Cho, Sukhyeong | * |
dc.author.google | Jeong, Kijun | * |
dc.author.google | Park, Jinbyung | * |
dc.author.google | Lee, Jinwon | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.date.modifydate | 20240322114808 | * |