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Identification of Secondary Metabolites from Marine Sediment-derived Two Streptomyces spp.

Title
Identification of Secondary Metabolites from Marine Sediment-derived Two Streptomyces spp.
Authors
윤지수
Issue Date
2017
Department/Major
대학원 화학·나노과학과
Publisher
이화여자대학교 대학원
Degree
Master
Advisors
남상집
Abstract
More than 70% surface of earth is covered with the oceans and diverse marine organisms harbor in the oceans. Marine organisms living in the various environments have captivated researchers to explore novel marine natural products which could be developed into pharmaceutical agents. A number of biologically active natural products have been discovered from marine microorganisms. Therefore Marine microorganisms have been considered as remarkable sources of new drug discovery. The actinomycetes, the major group of bacteria that produce potential secondary metabolites, are gram-positive bacteria that are common on terrestrial and in the ocean. The actinomycete bacteria are an abundant source of structurally interesting and pharmaceutically potential compounds, account for 45% of all microbial natural products. Marine-derived actinomycetes are being explored as a vastness source for unprecedented compounds. Streptomyces is the largest genus of the Actinobacteria, with over 500 species found predominantly in soil and marine ecosystems. To date, Streptomyces species are responsible for about two-thirds of the clinically used anti-biotics, and so investigations of Streptomyces spp. are of great interest to researchers. In this study, marine sediment-derived two Streptomyces spp. were explored to have unique chemical structures and bioactive marine natural products. In the course of investigation of bioactive secondary metabolites from the Streptomyces sp., three known compounds and two new compounds were elucidated. Ginseng greenberry were investigated to obtain novel natural products other than ginsenosides. In the course of investication of unique compound from ginseng greenberry, two compounds were isolated. Those compounds are first founded in the ginseng greenberry.;지구표면의 70% 이상은 바다로 둘러 싸여 있고 다양한 해양유기체들이 바다에서 서식하고 있다. 지상과는 다른 환경에서 서식하는 해양 유기체들은 선도 신약 물질이 될 수 있는 해양 천연물의 보고라고 할 수 있다. 생물학적으로 활성 있는 수많은 천연물 중에서 신규성 있는 물질들이 해양 미생물로부터 발견되었고, 해양미생물은 새로운 신약개발의 중요한 자원으로 여겨진다. 신규성 있는 이차대사산물을 생산하는 방선균은 토양이나 해양에서 흔히 발견되는 그람 양성균이며, 이 방선균으로부터 신규성있는 천연물45%가 생산되었다. 토양이나 해양에서 발견되는 500종이 넘는 Streptomyces sp.는 방선균의 가장 큰 종에 속하는데, 현재까지 치료개발에 사용되는 항생제의 3분의 2는 Streptomyces spp. 유래 물질이다. 이러한 이유로 선도물질 및 신약을 개발하기 위해Streptomyces sp를 연구하는 것은 매우 중요하다. 본 연구에서 해양유래 이종의 스트렙토마이시스가 생산해내는 이차대사산물 들을 분리하고 그 구조를 구명하였다. 스트렙토마이시스(SNA-042)로부터 4가지 알려진 물질인 sarmentosamide, tryptophan-dehydrobutyrine diketopiperazine, surugamide, 그리고 N-(7-hydroxy-3-oxo-4-cholen-24-oyl)-3-aminopropiomc acid 를 분리하였으며, 또 다른 Streptomyces 종(CNT-189)으로부터는 2종의 신규천연물 4Z- 12- dehydroxykromycin and 4E-12-dehydroxykromycin 구조를 규명하였다. 인삼 베리 성장단계 중 하나인 인삼 그린베리 열매로부터 처음으로 Tridecanedioic acid,1,13-diethyl ester, Tetradecanoic acid,14-(acethyloxy)-를 분리정제하였다.
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