View : 319 Download: 0

Effect of Human Placental Extract Treatment on Random-Pattern Skin Flap Survival in Rats

Title
Effect of Human Placental Extract Treatment on Random-Pattern Skin Flap Survival in Rats
Authors
Kwon, Jung WooHong, Seung EunKang, So RaPark, Bo Young
Ewha Authors
강소라박보영
SCOPUS Author ID
강소라scopus; 박보영scopus
Issue Date
2019
Journal Title
JOURNAL OF INVESTIGATIVE SURGERY
ISSN
0894-1939JCR Link

1521-0553JCR Link
Citation
JOURNAL OF INVESTIGATIVE SURGERY vol. 32, no. 4, pp. 304 - 313
Keywords
human placental extractrandom-pattern flapantioxidant
Publisher
TAYLOR &

FRANCIS INC
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Background: Human placental extract (HPE), prepared from the placentas of healthy, postpartum females, displays various physiological activities, including antioxidative properties. In this study, a dorsal skin flap model was used to investigate the effect of HPE on flap viability in rats. Materials and methods: Forty male Sprague-Dawley rats underwent random-pattern skin flap surgeries. The animals were randomly divided among a control group and three treatment groups (localized injection (LI), 10 mg/kg/d localized HPE injections; low-dose treatment (LT), 10 mg/kg/d systemic HPE injections; high-dose treatment (HT), 40 mg/kg/d systemic HPE injections). Surviving skin flap areas were measured 7 days after surgery and tissue samples were stained with hematoxylin and eosin; vascular endothelial growth factor expression was determined immunohistochemically. To evaluate the antioxidant and antiapoptotic effects of HPE, malondialdehyde, glutathione peroxidase, and caspase-3 levels were examined. Results: Seven days after surgery, HPE-treated animals had significantly reduced necrotic areas, rats receiving the highest HPE dose demonstrated the greatest flap survival. In the HPE groups, the histopathological scores were lower than for the control group. Immunohistochemistry showed markedly more numerous vascular endothelial growth factor-positive cells in the HT group than in the C group. Malondialdehyde levels were significantly lower and glutathione peroxidase levels were higher in the HT group than in the C group. HPE treatment significantly inhibited apoptosis by lowering caspase-3 activity. Conclusions: HPE treatment yielded positive effects on flap survival, due to its antioxidant and antiapoptotic properties. These results suggest a new therapeutic approach for enhancing flap viability and accelerating wound repair.
Show the fulltext
DOI
10.1080/08941939.2017.1417518
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE