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Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells
- Title
- Exogenous Hydrogen Peroxide Induces Lipid Raft-Mediated STAT-6 Activation in T Cells
- Authors
- Kim, Hee Ja; Lim, Jiwoo; Jang, Young-Soon; Shin, Eui-Cheol; Kim, Hyung-Ran; Seoh, Ju-Young; Lee, Ji Soo; Lee, Soon Nam; Kang, Jihee Lee; Choi, Youn-Hee
- Ewha Authors
- 서주영; 이지희; 이순남; 이지수; 최윤희
- SCOPUS Author ID
- 서주영; 이지희; 이순남; 이지수; 최윤희
- Issue Date
- 2017
- Journal Title
- CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
- ISSN
- 1015-8987
1421-9778
- Citation
- CELLULAR PHYSIOLOGY AND BIOCHEMISTRY vol. 42, no. 6, pp. 2467 - 2480
- Keywords
- Reactive oxygen species (ROS); IL-4; STAT-6; Lipid rafts; Th2 cells
- Publisher
- KARGER
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Background/Aims: CD4+ T cells are a critical component of the adaptive immune response. While the mechanisms controlling the differentiation of the Th1, Th17, and regulatory T cell subsets from naive CD4+ T cells are well described, the factors that induce Th2 differentiation are still largely unknown. Methods: The effects of treatment with exogenous H2O2 on STAT-6 phosphorylation and activation in T cells were examined by imnnunoblotting, imnnunofluorescence and gel shift assay. Anti-CD3 antibody and methyl-beta-cyclodextrin were utilized to induce lipid raft assembly and to investigate the involvement of lipid rafts, respectively. Results: Jurkat and EL-4T cells that were exposed to H2O2 showed rapid and strong STAT-6 phosphorylation, and the extent of STAT-6 phosphorylation was enhanced by co-treatment with anti-CD3 antibody. The effect of H2O2 on STAT-6 phosphorylation and translocation was inhibited by disruption of lipid rafts. STAT-6 activation in response to H2O2 treatment regulated IL-4 gene expression, and this response was strengthened by treatment with anti-CD3. Conclusion: Our results indicate that reactive oxygen species such as H2O2 can act on upstream and initiating factors for activation of STAT-6 in T cells and contribute to formation of a positive feedback loop between STAT-6 and IL-4 in the Th2 differentiation process. (C) 2017 The Author(s) Published by S. Karger AG, Basel
- DOI
- 10.1159/000480210
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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