Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이공주 | - |
dc.contributor.author | 신동해 | - |
dc.date.accessioned | 2017-11-01T04:29:55Z | - |
dc.date.available | 2017-11-01T04:29:55Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.other | OAK-21104 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/237848 | - |
dc.description.abstract | Secretagogin (SCGN), a hexa EF-hand calcium binding protein, plays key roles in insulin secretion in pancreatic β-cells. It is not yet understood how the binding of Ca2+ to human SCGN (hSCGN) promotes secretion. Here we have addressed this question, using mass spectrometry combined with a disulfide searching algorithm DBond. We found that the binding of Ca2+ to hSCGN promotes the dimerization of hSCGN via the formation of a Cys193-Cys193 disulfide bond. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics studies revealed that Ca2+ binding to the EF-hands of hSCGN induces significant structural changes that affect the solvent exposure of N-terminal region, and hence the redox sensitivity of the Cys193 residue. These redox sensitivity changes were confirmed using biotinylated methyl-3-nitro-4-(piperidin-1-ylsulfonyl) benzoate (NPSB-B), a chemical probe that specifically labels reactive cysteine sulfhydryls. Furthermore, we found that wild type hSCGN overexpression promotes insulin secretion in pancreatic β cells, while C193S-hSCGN inhibits it. These findings suggest that insulin secretion in pancreatic cells is regulated by Ca2+ and ROS signaling through Ca2+-induced structural changes promoting dimerization of hSCGN. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Calcium Ion Induced Structural Changes Promote Dimerization of Secretagogin, Which is Required for Its Insulin Secretory Function | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 7 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | Scientific Reports | - |
dc.identifier.doi | 10.1038/s41598-017-07072-4 | - |
dc.identifier.wosid | WOS:000406764200008 | - |
dc.identifier.scopusid | 2-s2.0-85026781907 | - |
dc.author.google | Lee J.-J. | - |
dc.author.google | Yang S.-Y. | - |
dc.author.google | Park J. | - |
dc.author.google | Ferrell J.E. | - |
dc.author.google | Jr. | - |
dc.author.google | Shin D.-H. | - |
dc.author.google | Lee K.-J. | - |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | - |
dc.contributor.scopusid | 신동해(57217374185) | - |
dc.date.modifydate | 20230208115507 | - |