View : 256 Download: 0
Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen
- Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen
- Jang, A-Yeung; Seo, Ho Seong; Lin, Shunmei; Chung, Gook-Hyun; Kim, Han Wool; Lim, Sangyong; Zhao, Lei; Park, In Ho; Lim, Jae Hyang; Kim, Kyung-Hyo
- Ewha Authors
- 김경효; 박인호; 임재향; 김한울
- SCOPUS Author ID
- 김경효; 임재향; 김한울
- Issue Date
- Journal Title
- VIRULENCE vol. 8, no. 6, pp. 875 - 890
- annexin A2; pneumococcal surface protein K; Streptococcus pneumoniae; vaccine
- TAYLOR &
- SCIE; SCOPUS
- Document Type
Show the fulltext
- The pneumococcal capsule is indispensable for pathogenesis in systemic infections; however, many pneumococcal diseases, including conjunctivitis, otitis media, and some systemic infections in immunocompromised patients, are caused by nonencapsulated Streptococcus pneumoniae (NESp). Null capsule clade 1 (NCC1), found in group 2 NESp, expresses pneumococcal surface protein K (PspK) and is becoming prevalent among pneumococcal organisms owing to the widespread use of pneumococcal conjugate vaccines. Despite its clinical importance, the molecular mechanisms underlying the prevalence of NCC1 have not been fully elucidated. Here, we investigated the role of the R3 domain of PspK in the epithelial cell adherence of NCC1. We found that the R3 domain of PspK mediated NCC1 adherence via its direct interaction with the epithelial surface protein annexin A2. Additionally, neutralization with purified recombinant PspK-R3 or rabbit anti-UD: R3 IgG inhibited binding of NESp to lung epithelial cells in vitro. Immunization with the 'repeat' domain of PspK-R3 or PspK-UD: R3 effectively elicited mucosal and systemic immune responses against PspK-R3 and provided protection against nasopharyngeal, lung, and middle ear colonization of NESp in mice. Additionally, we found that rabbit anti-UD: R3 IgG bound to PspC-R1 of the encapsulated TIGR4 strain and that UD: R3 immunization provided protection against nasopharyngeal and lung colonization of TIGR4 and deaths by TIGR4 and D39 in mice. Further studies using 68 pneumococcal clinical isolates showed that 79% of clinical isolates showed cross-reactivity to rabbit anti-UD: R3 IgG. About 87% of serotypes in the 13-valent pneumococcal conjugate vaccine (PCV) and 68% of non-vaccine serotypes were positive for cross-reactivity with rabbit anti-UD: R3 IgG. Thus, the R3 domain of PspK may be an effective vaccine candidate for both NESp and encapsulated Sp.
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.