Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김승정 | * |
dc.date.accessioned | 2017-08-25T05:08:13Z | - |
dc.date.available | 2017-08-25T05:08:13Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 2288-6575 | * |
dc.identifier.other | OAK-20914 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/235717 | - |
dc.description.abstract | Purpose: Neointimal hyperplasia (NH) is considered to be one of the main causes of vascular access occlusion in patients receiving hemodialysis. Endothelial injury and TGF-β-mediated proliferation of vascular smooth muscle cells (VSMCs) induce NH. Endothelial microparticles (EMPs) are also increased by endothelial injury. We aimed to investigate the effects of EMPs and TGF-β expression on VSMC proliferation and their contributions to NH formation in an ex vivo model. Methods: EMPs were collected from the culture media of human umbilical vein endothelial cells treated with indoxyl sulfate (IS, 250 μg/mL) after ultracentrifugation at 100,000 × g. Porcine internal jugular veins were isolated and treated with EMPs (2 × 106/mL) or left untreated for 12 days and subsequently compared with TGF-β (10 ng/mL)-treated venous tissue. Intima-media thickness and NH area were assessed using a digital program. Masson's trichrome staining and immunohistochemistry (IHC) analysis for α-smooth muscle actin, phosphorylated Akt, ERK1/2, p38 mitogen-activated protein kinase (MAPK), and Smad3 were performed on each vein sample. Results: NH and VSMC proliferation developed to a significantly greater degree in EMP-treated veins compared to controls, with similar patterns seen in TGF-β-stimulated samples. IHC analysis demonstrated that EMPs markedly increased phosphorylation of Akt, ERK1/2, p38 MAPK, and Smad3 in areas of venous NH formation. Conclusion: Our results showed that IS-induced EMPs provoked massive VSMC proliferation and NH formation via activation of the TGF-β signaling pathways. Further investigation is needed to elucidate the precise mechanism of EMP activity on vascular access stenosis in vivo. Copyright © 2017, the Korean Surgical Society. | * |
dc.language | English | * |
dc.publisher | Korean Surgical Society | * |
dc.subject | Cell-derived microparticles | * |
dc.subject | Neointima | * |
dc.subject | Transforming growth factor beta | * |
dc.title | The effects of indoxyl sulfate-induced endothelial microparticles on neointimal hyperplasia formation in an ex vivo model | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 93 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 11 | * |
dc.relation.lastpage | 17 | * |
dc.relation.journaltitle | Annals of Surgical Treatment and Research | * |
dc.identifier.doi | 10.4174/astr.2017.93.1.11 | * |
dc.identifier.wosid | WOS:000404504500002 | * |
dc.identifier.scopusid | 2-s2.0-85024116882 | * |
dc.author.google | Ryu J.-H. | * |
dc.author.google | Park H. | * |
dc.author.google | Kim S.-J. | * |
dc.contributor.scopusid | 김승정(8619054500) | * |
dc.date.modifydate | 20240419142141 | * |