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Vitamin D Insufficiency Exacerbates Adipose Tissue Macrophage Infiltration and Decreases AMPK/SIRT1 Activity in Obese Rats
- Title
- Vitamin D Insufficiency Exacerbates Adipose Tissue Macrophage Infiltration and Decreases AMPK/SIRT1 Activity in Obese Rats
- Authors
- Chang, Eugene; Kim, Yangha
- Ewha Authors
- 김양하; 장유진
- SCOPUS Author ID
- 김양하; 장유진
- Issue Date
- 2017
- Journal Title
- NUTRIENTS
- ISSN
- 2072-6643
- Citation
- NUTRIENTS vol. 9, no. 4
- Keywords
- adenosine monophosphate-activated protein kinase (AMPK); adipose tissue macrophage infiltration; obesity; sirtulin 1 (SIRT1); vitamin D
- Publisher
- MDPI AG
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Obesity is recognized as a state of chronic low-grade systemic inflammation due to adipose tissue macrophage infiltration and production of proinflammatory adipokines. Decreased vitamin D status is associated with obesity. The specific aim of the present study is to investigate the effects of vitamin D on obesity-induced adipose tissue inflammation. Male Sprague-Dawley rats were randomized and fed a normal diet (NOR, 1000 IU vitamin D/kg diet), a 45% high-fat diet (HF, 1000 IU vitamin D/kg diet), or a 45% high-fat diet containing 25 IU vitamin D/kg diet (HF+LVD) for 12 weeks. The vitamin D-insufficient diet (HF+LVD) led to vitamin D inadequacy as determined by serum 25(OH) D level, 68.56 +/- 7w.97 nmol/L. The HF+LVD group exacerbated HF-increased adipocyte size, adipogenic gene expression of PPAR gamma, adipose tissue macrophage recruitment, and proinflammatory cytokine IL-6 and TNF alpha levels in epididymal white adipose tissue. In addition, vitamin D insufficiency significantly decreased mRNA levels of beta-oxidation-related genes such as CPT1 alpha, PGC1 alpha, PPAR alpha, VLCAD, LCAD, MCAD, and UCP1. Moreover, significant decrements of SIRT1 and AMPK activity were noted in obese rats fed with a vitamin D-insufficient diet. The observed deleterious effects of vitamin D insufficiency on adipose tissue expansion, immune cell infiltration and inflammatory status suggest vitamin D plays a beneficial role in adipocyte metabolic metabolism and obesity progression. SIRT1 and AMPK activity may play a role in the mechanism of vitamin D action.
- DOI
- 10.3390/nu9040338
- Appears in Collections:
- 신산업융합대학 > 식품영양학과 > Journal papers
- Files in This Item:
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Vitamin D Insufficiency Exacerbates Adipose Tissue.pdf(1.19 MB)
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