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dc.contributor.author배윤수*
dc.date.accessioned2017-02-15T08:02:32Z-
dc.date.available2017-02-15T08:02:32Z-
dc.date.issued2007*
dc.identifier.issn0898-6568*
dc.identifier.otherOAK-4147*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/234405-
dc.description.abstractPhospholipase C-γ1 (PLC-γ1), which generates two second messengers, namely, inositol-1, 4, 5-trisphosphate and diacylglycerol, is implicated in growth factor-mediated chemotaxis. However, the exact role of PLC-γ1 in integrin-mediated cell adhesion and migration remains poorly understood. In this study, we demonstrate that PLC-γ1 is required for actin cytoskeletal organization and cell motility through the regulation of Pyk2 and paxillin activation. After fibronectin stimulation, PLC-γ1 directly interacted with the cytoplasmic tail of integrin β1. In PLC-γ1-silenced cells, integrin-induced Pyk2 and paxillin phosphorylation were significantly reduced and PLC-γ1 potentiated the integrin-induced Pyk2/paxillin activation in its enzymatic activity-dependent manner. In addition, specific knock-down of PLC-γ1 resulted in a failure to form focal adhesions dependent on fibronectin stimulation, which appeared to be caused by the suppression of Pyk2 and paxillin phosphorylation. Interestingly, PLC-γ1 potentiated the activations of Rac, thus integrin-induced lamellipodia formation was up-regulated. Consequently, the strength of cell-substratum interaction and cell motility were profoundly up-regulated by PLC-γ1. Taken together, these results suggest that PLC-γ1 is a key player in integrin-mediated cell spreading and motility achieved by the activation of Pyk2/paxillin/Rac signaling. © 2007 Elsevier Inc. All rights reserved.*
dc.languageEnglish*
dc.titlePhospholipase C-γ1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation*
dc.typeArticle*
dc.relation.issue8*
dc.relation.volume19*
dc.relation.indexSCI*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage1784*
dc.relation.lastpage1796*
dc.relation.journaltitleCellular Signalling*
dc.identifier.doi10.1016/j.cellsig.2007.04.002*
dc.identifier.wosidWOS:000248175200017*
dc.identifier.scopusid2-s2.0-34250639027*
dc.author.googleChoi J.H.*
dc.author.googleYang Y.-R.*
dc.author.googleLee S.K.*
dc.author.googleKim I.-S.*
dc.author.googleHa S.H.*
dc.author.googleKim E.-K.*
dc.author.googleBae Y.S.*
dc.author.googleRyu S.H.*
dc.author.googleSuh P.-G.*
dc.contributor.scopusid배윤수(15031067200)*
dc.date.modifydate20240415133331*
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자연과학대학 > 생명과학전공 > Journal papers
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