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dc.contributor.author신윤용-
dc.date.accessioned2017-02-15T08:02:56Z-
dc.date.available2017-02-15T08:02:56Z-
dc.date.issued2007-
dc.identifier.issn0048-9697-
dc.identifier.otherOAK-3800-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/234232-
dc.description.abstractCALUX, Chemically Activated LUciferase gene eXpression bioassay, has proven valuable for screening for and assessing toxic equivalents of dioxin-like compounds, because it detects all AhR (arylhydrocarbon receptor) ligands in a variety of sample matrices. In this study, we tried to validate DRE (dioxin-response elements)-CALUX bioassay, which has been developed by cloning mouse cyp1a1 gene in front of luciferase reporter gene. We compared DRE-CALUX bioassay with high resolution gas chromatography/mass spectrometry (HRGC/MS) for assessing environmental samples from Korea. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dose response study showed high correlation (r2 = 0.89) between DRE-CALUX bioassay and EROD (ethoxyresorufin O-deethylase) bioassay, a commonly used bioassay method. The mean TEQ value of water samples was 0.57 pg-TEQHRGC/MS/L and 4.97 pg-TEQCALUX/L. For soil samples, HRGC/MS-TEQ values ranged from 0 to 47.18 pg-TEQ/g (dry) and correlated well (r2 = 0.98) with values obtained by CALUX-TEQ which ranged from 0.92 to 649.97 pg-TEQ/g (dry). The difference between the absolute TEQ values might be due to the presence of dioxin-like compounds without WHO-TEQ values rather than the difference between CALUX-REP and WHO-TEQ. Based on this study, we suggest that DRE-CALUX bioassay can serve as an alternative bioassay method for high-throughput analysis of large number of environmental samples. © 2006 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.titleDRE-CALUX bioassay in comparison with HRGC/MS for measurement of toxic equivalence in environmental samples-
dc.typeArticle-
dc.relation.issue41308-
dc.relation.volume372-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage657-
dc.relation.lastpage667-
dc.relation.journaltitleScience of the Total Environment-
dc.identifier.doi10.1016/j.scitotenv.2006.10.036-
dc.identifier.wosidWOS:000243797300030-
dc.identifier.scopusid2-s2.0-33846005824-
dc.author.googleJoung K.E.-
dc.author.googleChung Y.H.-
dc.author.googleSheen Y.Y.-
dc.contributor.scopusid신윤용(6603872711)-
dc.date.modifydate20210301081004-
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약학대학 > 약학과 > Journal papers
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