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Pim-1 induced polyploidy but did not affect megakaryocytic differentiation of K562 cells and CD34+ cells from cord blood
- Title
- Pim-1 induced polyploidy but did not affect megakaryocytic differentiation of K562 cells and CD34+ cells from cord blood
- Authors
- Jung Y.-J.; Chae H.-C.; Seoh J.-Y.; Ryu K.-H.; Park H.-K.; Kim Y.-J.; Woo S.-Y.
- Ewha Authors
- 박혜경; 서주영; 김영주; 유경하; 우소연
- SCOPUS Author ID
- 박혜경; 서주영; 김영주; 유경하; 우소연
- Issue Date
- 2007
- Journal Title
- European Journal of Haematology
- ISSN
- 0902-4441
- Citation
- European Journal of Haematology vol. 78, no. 2, pp. 131 - 138
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- In a previous study, we determined the gene expression profile of both megakaryocytic and non-megakaryocytic lineage cells via serial analysis of gene expression and microarray methods, and demonstrated that Pim-1 was expressed more abundantly in megakaryocytic lineage cells. In this study, we knocked down Pim-1 in K562 cells, as well as in CD34+ cells from cord blood, via RNA interference, in order to analyze the effects of Pim-1 expression on the megakaryocytic differentiation of these cells. We then additionally overexpressed the Pim-1 genes in K562 cells, and conducted a comparison of these effects with those of RNAi cells on the course of megakaryocytic differentiation. The results of this study revealed that Pim-1 knockdown exerted no effects on commitment or differentiation toward megakaryocytic lineage, as evidenced by the detected CD41+ or CD61+ cells, or on the number of megakaryocytic colony forming units. However, Pim-1 knockdown was found to elicit a reduction in CD41+ cells with >4n DNA content, and a concomitant increase in the fraction of cells achieving a ploidy of >4n in the Pim-1 overexpressing population of K562 cells. Collectively, the findings of these studies indicate that the expression of Pim-1 expression is both necessary and sufficient for polyploidization, but is not critical to cytoplasmic differentiation on megakaryopoiesis. © 2007 Blackwell Munksgaard.
- DOI
- 10.1111/j.1600-0609.2006.00791.x
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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