Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2017-02-15T08:02:33Z | - |
dc.date.available | 2017-02-15T08:02:33Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.other | OAK-3569 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234117 | - |
dc.description.abstract | Tylosin polyketide synthase (Tyl PKS) was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster using two compatible low-copy plasmids, each under the control of a pikAI promoter. The mutant strain produced 0.5 mg/l of the 16-membered ring macrolactone, tylactone, after a 4-day culture, which is a considerably reduced culture period to reach the maximum production level compared to other Streptomyces hosts. To improve the production level of tylactone, several precursors for ethylmalonyl-CoA were fed to the growing medium, leading to a 2.8-fold improvement (1.4 mg/ml); however, switching the pikAI promoter to an actI promoter had no observable effect. In addition, a small amount of desosamine-glycosylated tylactone was detected from the extract of the mutant strain, revealing that the native glycosyltransferase DesVII displayed relaxed substrate specificity in accepting the 16-membered ring macrolactone to produce the glycosylated tylactone. These results demonstrate a successful attempt for a heterologous expression of Tyl PKS in S. venezuelae and introduce S. venezuelae as a rapid heterologous expression system for the production of secondary metabolites. © 2006 Springer-Verlag. | - |
dc.language | English | - |
dc.title | Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 72 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 763 | - |
dc.relation.lastpage | 769 | - |
dc.relation.journaltitle | Applied Microbiology and Biotechnology | - |
dc.identifier.doi | 10.1007/s00253-006-0318-5 | - |
dc.identifier.wosid | WOS:000240809600019 | - |
dc.identifier.scopusid | 2-s2.0-33748999758 | - |
dc.author.google | Jung W.S. | - |
dc.author.google | Lee S.K. | - |
dc.author.google | Hong J.S.J. | - |
dc.author.google | Park S.R. | - |
dc.author.google | Jeong S.J. | - |
dc.author.google | Han A.R. | - |
dc.author.google | Sohng J.K. | - |
dc.author.google | Kim B.G. | - |
dc.author.google | Choi C.Y. | - |
dc.author.google | Sherman D.H. | - |
dc.author.google | Yoon Y.J. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |