Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2017-01-18T02:01:36Z | - |
dc.date.available | 2017-01-18T02:01:36Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.other | OAK-4281 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234030 | - |
dc.description.abstract | A method employing silicone oil density centrifugation, solid-phase extraction (SPE) cleanup, and LC-ESI-MS/MS analysis was developed for the rapid, selective, sensitive, and quantitative detection of an intracellular pool of short organic acid-CoA esters in actinomycetes. The detection limit was determined to be approximately 0.8 pmol (1.2 ng/ml) for each standard CoA-ester analyzed by the present LC-ESI-MS/MS method. A selected ion chromatogram for a typical fragment ion (m/z 428) specific to CoA-esters enabled the detection of eight intracellular CoA-esters involved in both primary and secondary metabolisms. The application of this method to bacterial metabolomic study is demonstrated by the profiling of the intracellular CoA-ester pools in the wild-type Streptomyces venezuelae strain producing polyketide antibiotics (methymycin and pikromycin), a polyketide synthase (PKS)-deleted S. venezuelae mutant, and a S. venezuelae mutant expressing the heterologous PKS genes. By quantifying the individual CoA-esterlevel in three different genotypes of the S. Venezuela e strain, further insight could be gained into the role of CoA-estersin polyketide biosynthesis. This analytical approach can be extended to the quantification of the size and composition of in vivo CoA-ester pools in various microbes, and can provide a detailed understanding of the relationship between the in vivo CoA-ester pool and the production of pharmaceutically important polyketides. Copyright © 2007 John Wiley & Sons, Ltd. | - |
dc.language | English | - |
dc.title | Analysis of intracellular short organic acid-coenzyme a esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry | - |
dc.type | Article | - |
dc.relation.issue | 9 | - |
dc.relation.volume | 42 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1136 | - |
dc.relation.lastpage | 1147 | - |
dc.relation.journaltitle | Journal of Mass Spectrometry | - |
dc.identifier.doi | 10.1002/jms.1240 | - |
dc.identifier.wosid | WOS:000249463000002 | - |
dc.identifier.scopusid | 2-s2.0-34548635755 | - |
dc.author.google | Je W.P. | - |
dc.author.google | Won S.J. | - |
dc.author.google | Sung R.P. | - |
dc.author.google | Byoung C.P. | - |
dc.author.google | Yeo J.Y. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |