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Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo

Title
Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo
Authors
Lee J.-Y.Choo J.-E.Choi Y.-S.Park J.-B.Min D.-S.Lee S.-J.Rhyu H.K.Jo I.-H.Chung C.-P.Park Y.-J.
Ewha Authors
이승진조인호
SCOPUS Author ID
이승진scopus; 조인호scopusscopus
Issue Date
2007
Journal Title
Biomaterials
ISSN
0142-9612JCR Link
Citation
Biomaterials vol. 28, no. 29, pp. 4257 - 4267
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration. © 2007 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2007.05.040
Appears in Collections:
약학대학 > 약학과 > Journal papers
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