Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차선신 | * |
dc.date.accessioned | 2016-12-28T02:12:26Z | - |
dc.date.available | 2016-12-28T02:12:26Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1225-8873 | * |
dc.identifier.issn | 1976-3794 | * |
dc.identifier.other | OAK-18766 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233281 | - |
dc.description.abstract | Chaperonins (CPNs) are megadalton sized ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation. They consist of two back-to-back stacked multisubunit rings. CPNs are usually classified into Group I and Group II. Here, we report the crystallization of both the AMPPNP (an ATP analogue) and ADP bound forms of a novel CPN, classified as belonging to a third Group, recently discovered in the extreme thermophile Carboxydothermus hydrogenoformans. Crystals of the two forms were grown by the vapor batch crystallization method at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 angstrom resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 angstrom resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813 angstrom. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%. | * |
dc.language | English | * |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | * |
dc.subject | Group III chaperonins | * |
dc.subject | protein folding | * |
dc.subject | ATP-fueled nanomachine | * |
dc.subject | crystallization | * |
dc.title | Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 54 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 440 | * |
dc.relation.lastpage | 444 | * |
dc.relation.journaltitle | JOURNAL OF MICROBIOLOGY | * |
dc.identifier.doi | 10.1007/s12275-016-6089-5 | * |
dc.identifier.wosid | WOS:000376653000006 | * |
dc.identifier.scopusid | 2-s2.0-84969919749 | * |
dc.author.google | An, Young Jun | * |
dc.author.google | Rowland, Sara E. | * |
dc.author.google | Robb, Frank T. | * |
dc.author.google | Cha, Sun-Shin | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.date.modifydate | 20240429134916 | * |