Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이서구 | * |
dc.contributor.author | 정우진 | * |
dc.date.accessioned | 2016-08-29T11:08:45Z | - |
dc.date.available | 2016-08-29T11:08:45Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0003-2697 | * |
dc.identifier.other | OAK-5821 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232027 | - |
dc.description.abstract | 2-Cys peroxiredoxin (Prx) is the major subgroup of a family of Prx enzymes that reduce peroxide molecules such as hydrogen peroxide (H2O2). 2-Cys Prxs are inactivated when their active site cysteine residue is hyperoxidized to sulfinic acid. Sulfiredoxin (Srx) is an enzyme that catalyzes reduction of hyperoxidized 2-Cys Prxs in the presence of ATP, Mg2+, and thiol equivalent. Therefore, Srx activity is crucial for cellular function of 2-Cys Prxs. The method currently available for the determination of Srx activity relies on immunoblot detection using antibodies to hyperoxidized enzymes. Here we introduce a simple quantitative assay for Srx activity based on the colorimetric determination of inorganic phosphate released in Srx-dependent reduction of hyperoxidized Prx using the malachite green. The colorimetric assay was used for high-throughput screening of 25,000 chemicals to find Srx inhibitors. © 2009 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.title | A colorimetric assay for sulfiredoxin activity using inorganic phosphate measurement | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 393 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 36 | * |
dc.relation.lastpage | 40 | * |
dc.relation.journaltitle | Analytical Biochemistry | * |
dc.identifier.doi | 10.1016/j.ab.2009.06.030 | * |
dc.identifier.wosid | WOS:000268731300005 | * |
dc.identifier.scopusid | 2-s2.0-67650976330 | * |
dc.author.google | Kim H. | * |
dc.author.google | Hong S. | * |
dc.author.google | Rhee S.G. | * |
dc.author.google | Jeong W. | * |
dc.contributor.scopusid | 이서구(7401852092) | * |
dc.contributor.scopusid | 정우진(35914322500) | * |
dc.date.modifydate | 20240423081003 | * |