Allelopathic effects of aqueous extracts from Eupatorium rugosum Houtt. and erigeron annus L. on radicles growth of Lactuca sativa and Raphanus raphanistroides

Abstract

We studied the allelopathic effects of aqueous extracts of Eupatorium rugosum Houtt. and Erigeron annus L. leaves on root hair development and electrophoretic protein patterns in Lactuca sativa L. and Raphanus raphanistroides Nakai. Root hair development in both test plants (L. sativa and R. raphanistroides) was inhibited by increasing donor plants aqueous extract concentration. Extracted proteins in radicles of test species were identified through SDS-PAGE. Protein bands of L. sativa treated with E. rugosum disappeared in the range of 26 to 18.5 kDa and those of L. sativa treated with E. annus disappeared in the range of 26 to 9 kDa. In R. raphanistroides, the protein bands were similar in control and treated plants. The results suggested that although the aqueous extracts from E. rugosum and E. annus inhibited the early growth of receptor plants, but their inhibitory allelopathic effects were stronger on L. sativa than on R. raphanistroides.