Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 김희선 | * |
dc.contributor.author | 박진선 | * |
dc.contributor.author | 이은정 | * |
dc.date.accessioned | 2016-08-29T12:08:59Z | - |
dc.date.available | 2016-08-29T12:08:59Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0021-8561 | * |
dc.identifier.other | OAK-14772 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230512 | - |
dc.description.abstract | Ginsenoside Rh3 is a bacterial metabolite of Rg5, which is the main constituent of heat-processed ginseng. The present study was undertaken to examine the anti-inflammatory effect of ginsenoside Rh3 in lipopolysaccharide (LPS)-stimulated microglia. Rh3 inhibits the expressions of inducible nitric oxide synthase (iNOS) and proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, at mRNA and protein levels, while Rh3 enhanced anti-inflammatory hemeoxygenase-1 expression. Moreover, Rh3 inhibited nuclear factor-κB (NF-κB) by upregulation of sirtuin 1 (SIRT1) and enhanced Nrf2 DNA-binding activities. Analysis of signaling pathways revealed that Rh3 enhanced the phosphorylation of 5′-adenosine monophosphate-activated protein kinase (AMPK) and inhibited Akt and janus kinase 1 (JAK1)/signal transducer and activator of transcription 1 (STAT1) induced by LPS. By treatment of BV2 cells with AICAR (a pharmacological activator of AMPK), we found that AMPK is an upstream regulator of phosphatidylinositol 3-kinase (PI3K)/Akt and JAK1/STAT1. Furthermore, AMPK knockdown experiments demonstrated the anti-inflammatory role of AMPK in LPS/Rh3-treated BV2 microglia. Our data collectively suggest that Rh3 exerts an anti-inflammatory effect in microglia by modulating AMPK and its downstream signaling pathways. © 2015 American Chemical Society. | * |
dc.language | English | * |
dc.publisher | American Chemical Society | * |
dc.subject | AMPK | * |
dc.subject | Ginsenoside Rh3 | * |
dc.subject | Microglia | * |
dc.subject | Neuroinflammation | * |
dc.subject | Signaling molecules | * |
dc.title | Anti-inflammatory Mechanism of Ginseng Saponin Metabolite Rh3 in Lipopolysaccharide-Stimulated Microglia: Critical Role of 5′-Adenosine Monophosphate-Activated Protein Kinase Signaling Pathway | * |
dc.type | Article | * |
dc.relation.issue | 13 | * |
dc.relation.volume | 63 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 3472 | * |
dc.relation.lastpage | 3480 | * |
dc.relation.journaltitle | Journal of Agricultural and Food Chemistry | * |
dc.identifier.doi | 10.1021/jf506110y | * |
dc.identifier.wosid | WOS:000352751000017 | * |
dc.identifier.scopusid | 2-s2.0-84926612579 | * |
dc.author.google | Lee Y.Y. | * |
dc.author.google | Park J.-S. | * |
dc.author.google | Lee E.-J. | * |
dc.author.google | Lee S.-Y. | * |
dc.author.google | Kim D.-H. | * |
dc.author.google | Kang J.L. | * |
dc.author.google | Kim H.-S. | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.contributor.scopusid | 박진선(54914743600) | * |
dc.contributor.scopusid | 이은정(57212665881) | * |
dc.date.modifydate | 20240215165648 | * |