Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김옥빈 | * |
dc.date.accessioned | 2016-08-28T11:08:14Z | - |
dc.date.available | 2016-08-28T11:08:14Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0021-9193 | * |
dc.identifier.other | OAK-13405 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/229404 | - |
dc.description.abstract | Escherichia coli K-12 is able to grow under aerobic conditions on D-malate using DctA for D-malate uptake and the D-malate dehydrogenase DmlA (formerly YeaU) for converting D-malate to pyruvate. Induction of dmlA encoding DmlA required an intact dmlR (formerly yeaT) gene, which encodes DmlR, a LysR-type transcriptional regulator. Induction of dmlA by DmlR required the presence of D-malate or L- or meso-tartrate, but only D-malate supported aerobic growth. The regulator of general C 4-dicarboxylate metabolism (DcuS-DcuR two-component system) had some effect on dmlA expression. The anaerobic L-tartrate regulator TtdR or the oxygen sensors ArcB-ArcA and FNR did not have a major effect on dmlA expression. DmlR has a high level of sequence identity (49%) with TtdR, the L- and meso-tartrate-specific regulator of L-tartrate fermentation in E. coli. dmlA was also expressed at high levels under anaerobic conditions, and the bacteria had D-malate dehydrogenase activity. These bacteria, however, were not able to grow on D-malate since the anaerobic pathway for D-malate degradation has a predicted yield of ≤0 ATP/mol D-malate. Slow anaerobic growth on D-malate was observed when glycerol was also provided as an electron donor, and D-malate was used in fumarate respiration. The expression of dmlR is subject to negative autoregulation. The network for regulation and coordination of the central and peripheral pathways for C 4- dicarboxylate metabolism by the regulators DcuS-DcuR, DmlR, and TtdR is discussed. Copyright © 2010, American Society for Microbiology. All Rights Reserved. | * |
dc.language | English | * |
dc.title | Regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (YeaT) | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 192 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2503 | * |
dc.relation.lastpage | 2511 | * |
dc.relation.journaltitle | Journal of Bacteriology | * |
dc.identifier.doi | 10.1128/JB.01665-09 | * |
dc.identifier.scopusid | 2-s2.0-77952070971 | * |
dc.author.google | Lukas H. | * |
dc.author.google | Reimann J. | * |
dc.author.google | Kim O.B. | * |
dc.author.google | Grimpo J. | * |
dc.author.google | Unden G. | * |
dc.contributor.scopusid | 김옥빈(15851079900) | * |
dc.date.modifydate | 20240301081003 | * |