Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김소연 | - |
dc.date.accessioned | 2016-08-28T11:08:07Z | - |
dc.date.available | 2016-08-28T11:08:07Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1976-9571 | - |
dc.identifier.other | OAK-13331 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/229341 | - |
dc.description.abstract | The Bombyx mori (B. mori) serine protease-like protein (BmSp) coding region (946 bp, GenBank accession number of mRNA, DQl 18520; protein, AAZ40503) was generated from two separate and overlapping cDNA fragments using sequence homology with Trichoplusia ni azurocidin in a Bombyx EST database (Silkbase! httpV/www.ab.a.u-tokyo.ac.jp/silkbase/). The deduced amino acid sequence of BmSp, which encodes 303 amino acids, shows 44% amino acid identity to A gambiae serine protease (CAA89967), 43% amino acid identity to Sarcophagi peregrina 26-kDa protease, an antibacterial protein and 31% identity to B. mon serine protease-2 (BmSP-2), a potential antiviral protein. Typical features of the BmSp included the serine protease active site triad His / Asp / Ser, three pairs of cysteine residues for disulfide bridges, and three residues, Asp / Gly / Gly, that help to confer trypsin-like specificity to the enzymes. Based on the result of sequence comparison and characterization, our results suggest that the BmSp probably the new subfamily of trypsin-like serine protease. Using RT-PCR and enzyme digestion, the full encoding sequence for BmSp was cloned into the E. coli expression vector pGEX-5X-l. The fusion protein GST-BmSp was effectively expressed in E. coli BL2l(DE3) pLysS as inclusion bodies, and a denaturation and refolding procedure were performed to obtain soluble GST-BmSp. The purified protein was tested for antibacterial activity against Gram-positive and Gram-negative bacteria, but it did not show antibacterial activity in the agar well diffusion assay and liquid growth inhibition assay. | - |
dc.language | English | - |
dc.title | Molecular cloning and characterization of a serine protease-like protein from silkworm (Bombyx Mon) | - |
dc.type | Article | - |
dc.relation.issue | 5 | - |
dc.relation.volume | 31 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 387 | - |
dc.relation.lastpage | 395 | - |
dc.relation.journaltitle | Genes and Genomics | - |
dc.identifier.doi | 10.1007/BF03191257 | - |
dc.identifier.wosid | WOS:000271933800007 | - |
dc.identifier.scopusid | 2-s2.0-71849119646 | - |
dc.author.google | Kim S.Y. | - |
dc.author.google | Jeong E.-J. | - |
dc.author.google | Song K.-J. | - |
dc.author.google | Park K.S. | - |
dc.date.modifydate | 20211001081000 | - |